Preparation of nasal secretions for proteome analysis.

Preparation of nasal secretions for proteome analysis. Research Abstract Details 

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Preparation of nasal secretions for proteome analysis. Abstract Text:

Begona Casado,Paolo Iadarola,Lewis K Pannell,

The determination of protein patterns in nasal secretions of healthy subjects can help in the early diagnosis of diseases such as acute sinusitis. The comparison of nasal lavage fluid collected from subjects with acute sinusitis before and after pharmacological treatment gives information about the drug effects on glandular secretions. Nasal secretions were stimulated with 1x NS (0.9% Normal Saline) and 24x NS in healthy subjects and in sinusitis subjects before and after pharmacological treatment. The nasal lavage fluid (NLF) proteins are precipitated with a solution of "acid-ethanol." Using this solution, the high molecular weight proteins precipitate and separate from the low molecular weight proteins. The proteins are digested and the peptides are separated using a capillary liquid chromatographic system. Eluted peptides are analyzed on ESI-Q-TOF mass spectrometry instrument.

Preparation of nasal secretions for proteome analysis. Publishing Authors By Initials

B Casado,P Iadarola,LK Pannell,

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Preparation of nasal secretions for proteome analysis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 425

Page Numbers: 77-87

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 28

MONTH: 03

YEAR: 2008

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LANGUAGE: eng

NlmUniqueID: 9214969

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AFFILIATION: Swiss Federal Institute of Technology, Zurich, Switzerland.

Country: United States

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MEDLINETA: Methods Mol Biol

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