Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate.

Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate. Research Abstract Details 

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Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate. Abstract Text:

Ittipon Saichana,Yoshitaka Ano,Osao Adachi,Kazunobu Matsushita,Hirohide Toyama,Ittipon Saichana,Yoshitaka Ano,Osao Adachi,Kazunobu Matsushita,Hirohide Toyama,

For easy measurement of 5-keto D-gluconate (5KGA) and 2-keto D-gluconate (2KGA), two enzymes, 5KGA reductase (5KGR) and 2KGA reductase (2KGR) are useful. The gene for 5KGR has been reported, and a corresponding gene was found in the genome of Gluconobacter oxydans 621H and was identified as GOX2187. On the other hand, the gene for 2KGR was identified in this study as GOX0417 from the N-terminal amino acid sequence of the partially purified enzyme. Several plasmids were constructed to express GOX2187 and GOX0417, and the final constructed plasmids showed good expression of 5KGR and 2KGR in Escherichia coli. From the two E. coli transformants, large amounts of each enzyme were easily prepared after one column chromatography, and the preparation was ready to use for quantification of 5KGA or 2KGA.

Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate. Publishing Authors By Initials

I Saichana,Y Ano,O Adachi,K Matsushita,H Toyama,I Saichana,Y Ano,O Adachi,K Matsushita,H Toyama,

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Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Bioscience, biotechnology, and biochemistry

VOLUME: 71

Page Numbers: 2478-86

Journal Abbreviation: Biosci. Biotechnol. Biochem.

ISSN: 0916-8451

DAY: 7

MONTH: 10

YEAR: 2007

Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate. Information

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LANGUAGE: eng

NlmUniqueID: 9205717

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Grant and Affiliation Information for Preparation of enzymes required for enzymatic quantification of 5-keto-D-gluconate and 2-keto-D-gluconate.

AFFILIATION: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan.

Country: Japan

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MEDLINETA: Biosci Biotechnol Biochem

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