[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA]

[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Abstract Text:

Yuan Li,Guosheng Dou,

Plasmid is a kind of popular nonviral vector in gene therapy. To produce large quantities of plasmid, an efficient large-scale purification process needs to be developed. In general, liquid chromatography is the most important tool for the analytical and preparative separation of plasmid DNA. In this study, biporous polymeric microspheres coupled with mercaptopyridine were synthesized for the purification of plasmid DNA. From the results of experiments, it can be inferred that the mechanism for the purification of the plasmid DNA with MP-BiPR is based on the hydrophobic interaction. When the load was 10 mL with the content of plasmid of 0.30 mg/mL and the content of RNA of 0.44 mg/mL, a flow rate was set up to 4 mL/min (20 cm/min). The separation was finished in 10 min, and the plasmid was completely recovered with undetectable impurities of nucleic acids and proteins. The load of the column for RNA was up to 2.2 mg/mL.

[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Publishing Authors By Initials

Y Li,G Dou,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Se pu = Chinese journal of chromatography / Zhongg

VOLUME: 25

Page Numbers: 820-4

Journal Abbreviation:

ISSN: 1000-8713

DAY: 8

MONTH: Nov

YEAR: 2007

[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 9424804

[Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA]

AFFILIATION: School of Material Science and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300130, China. wwwlycj@163.com

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Se Pu

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA Related Publications

• A caprine chimera produced by injection of embryonic germ cells into a blastocyst.
• A novel prodrug of the green tea polyphenol (-)-epigallocatechin-3-gallate as a potential anticancer agent.
• Determination of benzene, toluene, ethylbenzene, xylenes in water at sub-ngl(-1) levels by solid-phase microextraction coupled to cryo-trap gas chromatography-mass spectrometry.
• Effect of Bcl-2 and Bax on survival of side population cells from hepatocellular carcinoma cells.
• Evaluation of copper-dependent proteasome-inhibitory and apoptosis-inducing activities of novel pyrrolidine dithiocarbamate analogues.
• Identification and characterization of peptide mimics of blood group A antigen.
• Inhibition of proteasome activity by the dietary flavonoid apigenin is associated with growth inhibition in cultured breast cancer cells and xenografts.
• Inhibition of the proteasome activity by gallium(III) complexes contributes to their anti prostate tumor effects.
• Inhibitory Mg-ADP-Fluoroaluminate Complexes Bound to Catalytic Sites of F1-ATPases: Are They Ground-State or Transition-State Analogs?
• Lessons learned from Art Pardee in cell cycle, science, and life.
• New uses for old copper-binding drugs: converting the pro-angiogenic copper to a specific cancer cell death inducer.
• Pristimerin induces apoptosis by targeting the proteasome in prostate cancer cells.
• Structure-proteasome-inhibitory activity relationships of dietary flavonoids in human cancer cells.
• Tea polyphenols, their biological effects and potential molecular targets.
• The tumor proteasome is a primary target for the natural anticancer compound Withaferin A isolated from "Indian winter cherry".
• [A family with familial dysalbuminaemic hyperthyroxinaemia]
• [Biotherapy of Cancer by Anti-EGFR Monoclonal Antibody - Review.]
• [Effects of urbanization on supply and demand of regional ecological footprint]
• [Expression, purification and activity analysis of human single-chain antibody against hepatocellular cancer]
• [Gastrointestinal biomechanics--new perspectives in motility research]
• [Immunotherapy of non-Hodgkin's lymphomas (NHL) by anti-CD22 antibody--review]
• [Isolation, purification and identification of epithelial cells derived from fetal islet-like cell clusters]
• [Management of chylous leakage after radical operation of gastric cancer]
• [Near-infrared spectral studies of hydrogen-bond in water-methanol mixtures]
• [Preparation of biporous polymeric microspheres coupled with mercaptopyridine and its application in rapid purification of plasmid DNA]
• [Studies on the manufacture and immunogenicity of purified rabies vaccines on humans Vero cell]
• [Study on pluripotency and cultivation of ES-like cells derived from male germ stem cells of bovine fetuses]
• [T lymphocytes with chimeric receptor induce carcinoembryonic antigen-positive specific gastric carcinoma cells apoptosis]
• [The killing effect and bystander effect of linamarase/linamarin suicide gene system on human hepatocellular carcinoma cell line HepG2 in vitro.]
• [The origin and nature of embryonic stem cells]