Preparation of basement membrane components from EHS tumors.

Preparation of basement membrane components from EHS tumors. Research Abstract Details 

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Preparation of basement membrane components from EHS tumors. Abstract Text:

H K Kleinman,H K Kleinman,

This unit describes methods for passaging and harvesting the basement membrane matrix-producing EHS tumor and for the subsequent isolation of a crude mixture of basement membrane components termed Matrigel, which promotes the differentiation of a variety of epithelial, endothelial, and neuronal cells. Procedures for the isolation of the adhesive glycoprotein laminin-1 and of type IV collagen are also included. Support protocols cover the maintenance and harvesting of EHS tumors in mice and maintenance of mice on a lathrogenic diet.

Preparation of basement membrane components from EHS tumors. Publishing Authors By Initials

HK Kleinman,HK Kleinman,

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Preparation of basement membrane components from EHS tumors. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in cell biology / editorial boar

VOLUME: Chapter 10

Page Numbers: Unit 10.2

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ISSN: 1934-2616

DAY: 29

MONTH: May

YEAR: 2001

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LANGUAGE: eng

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AFFILIATION: National Institute of Dental Research/NIH, Bethesda, Maryland, USA.

Country: United States

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MEDLINETA: Curr Protoc Cell Biol

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