[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein]

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Abstract Text:

Yi Hu,Xue-Song Liu,Yong Zhu,Ying Liu,Xiao-Guang Xue,Jiang-Nan Xue,Bo-Quan Jin,

AIM: To prepare and characterize mAb to Fc fragment of Ig fusion protein, and to establish sandwich ELISA for detecting Ig fusion proteins and affinity chromatography method for Ig fusion protein purification. METHODS: hBCMA-Ig was used as antigen to immune BALB/c mice. The lymphocyte hybridoma technique was used to establish hybridoma cell lines stably secreting anti-Fc mAb. ELISA was employed to detect the isotype, epitopes, and species specificity of mAbs. Western blot was used to assess the reactivity between anti-Fc mAbs and denatured Ig fusion protein. LAIR1-Ig fusion protein was purified through affinity column anti-Fc mAb cross-linked to Sepharose 4B. RESULTS: Seven hybridoma cell lines(FMUFc1-FMUFc7) were acquired. A sandwich ELISA was successfully established using FMUFc4 as coating mAb and FMUFc5 as enzyme-labeled mAb. FMUFc6 could be used for Western blot. LAIR1-Ig fusion protein was effectively purified through FMUFc5-Sepharose affinity chromatography column. CONCLUSION: mAb against Fc fragment of Ig fusion protein has been prepared successfully, and methods to detect and purify Ig fusion protein are established. These mAbs provide useful tool for further application of Ig fusion protein.

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Publishing Authors By Initials

Y Hu,XS Liu,Y Zhu,Y Liu,XG Xue,JN Xue,BQ Jin,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 170-1

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 20

MONTH: Mar

YEAR: 2003

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 101139110

[Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein]

AFFILIATION: Deparment of Immunology, Fourth Military Medical University, Xi'an 710032, China.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein Related Publications

• AP-1 complexes mediate oxidized LDL-induced overproduction of TGF-beta(1) in rat mesangial cells.
• Daily short-term head-up tilt can prevent structural changes in arteries of tail-suspended rats.
• Urethral reconstruction with colonic mucosa in treatment of complex urethral stricture.
• [Animal models of schizophrenia using different laboratory mouse strains]
• [Apoptosis-inducing ligand TRAIL can be recruited to lipid rafts]
• [Application of BACTEC MGIT 960 system and molecular identification of mycobacteria in the diagnosis of spinal tuberculosis]
• [CD226 monoclonal antibody induces variation of intracytosolic free calcium level in human umbilical vein endothelial cells]
• [Cloning, prokaryotic expression of LAIR and identification of their immunological reactivities]
• [Development and preliminary application of a double mAb sandwich ELISA for detecting soluble CD226]
• [Development of conductance measurement technique for permeability of monolayer endothelial cells]
• [Diagnosis and treatment of methylmalonic acidemia in 14 cases]
• [Effect of low-intensity microwave of on mitomycin C-induced genotoxicity in vitro]
• [Effects of TGF beta on BMP-2 gene transfection cells in vitro]
• [Effects of human BMP2 gene transfection on NIH3T3 cells in vitro]
• [Effects of perindopril on plasma soluble TRAIL and receptor soluble DR5 in patients with congestive heart failure]
• [Establishment of a sandwich ELISA for detecting serum sTRAIL and its application]
• [Eukaryotic expression and application of human CD226 Ig fusion protein containing 3C protease-restricted site]
• [General development situation of space food for space station]
• [High polymorphism at the human P gene locus in Chinese Han and Tibetan populations]
• [Mitogenesis of platelet-derived growth factors to human osteoblasts modulated by basic fibroblast growth factor]
• [Morphological evidence on participating in adhesive function of human umbilical vein endothelial cells by CD226]
• [One case of congenital intranasal cephalocele]
• [Pioglitazone, a peroxisome proliferators-activated receptor gamma ligand, inhibits dimethylhydrazine (DMH) induced aberrant crypt foci in rats]
• [Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor]
• [Preparation, characterization and application of monoclonal antibodies to Fc fragment of Ig fusion protein]
• [Study on the detection of inv(16) in acute myelomonocytic leukemia (M4) by flucorescence in situ hybridization method]
• [The acute effects of dimethoate on the muscarinic-receptors of rat brains and the relationship between muscarinic-receptors and cholinesterase]
• [The combination of cyclosporin A and androgen in the treatment of chronic aplastic anemia]
• [The evaluation of LFH or LFPH in the treatment of advanced cancer of gastric cardia and colorectal cancer]
• [Treatment of childhood leukemia with unrelated donor allogeneic bone marrow transplantation]