Preparation and some properties of giant liposomes and proteoliposomes.

Preparation and some properties of giant liposomes and proteoliposomes. Research Abstract Details 

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Preparation and some properties of giant liposomes and proteoliposomes. Abstract Text:

K Higashi,S Suzuki,H Fujii,Y Kirino,

Optimal conditions for formation of giant liposomes and proteoliposomes were investigated. A suspension of small unilamellar vesicles made of various phospholipids in a buffer of 0-3 M KCl, 0.1 mM EDTA, and 20 mM MOPS (pH 7.0) was subjected to a freeze-thaw treatment. Giant multilamellar liposomes of diameter ranging from 10 to 60 microns were found to form from phospholipid mixtures containing phosphatidylethanolamine as a major component and phosphatidylserine as a minor component. The concentration of KCl optimal for the giant vesicle formation was 30-500 mM. By applying a patch-pipette to a giant liposome, suitable conditions for obtaining a high-resistance (giga-ohm) seal were sought. It was found that use of a patch-pipette of relatively small tip diameter (less than 1 micron), the presence of divalent metal cations in the suspension medium and inflation of vesicles in a hypotonic solution facilitated giga-seal formation. In a suspension of asolectin (soybean phospholipid) vesicles which had been subjected to the freeze-thaw treatment, giant unilamellar vesicles were found. They could be held on the tip of a suction pipette and impaled with a microelectrode filled with an EGTA solution. Small unilamellar proteoliposomes were prepared by the cholate-dialysis method from asolectin and sarcoplasmic reticulum vesicles, and were subjected to a freeze-thaw cycle. When the ratio of exogenous phospholipid to protein was larger than 10, giant multilamellar vesicles were formed.(ABSTRACT TRUNCATED AT 250 WORDS)

Preparation and some properties of giant liposomes and proteoliposomes. Publishing Authors By Initials

K Higashi,S Suzuki,H Fujii,Y Kirino,

For similar complex mixtures: colloids: suspensions research abstracts see: complex mixtures: colloids: suspensions research

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Preparation and some properties of giant liposomes and proteoliposomes. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 101

Page Numbers: 433-40

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1987

Preparation and some properties of giant liposomes and proteoliposomes. Information

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LANGUAGE: eng

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Preparation and some properties of giant liposomes and proteoliposomes. Keywords Mesh Terms:

KEYWORDS: Suspensions

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Chemical & Substance for Abstract: Preparation and some properties of giant liposomes and proteoliposomes. Information

Substance Name: proteoliposomes

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Country: JAPAN

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MEDLINETA: J Biochem

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