Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond.

Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Research Abstract Details 

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Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Abstract Text:

H Tamaoki,F Sakiyama,K Narita,

Ribonuclease T1 [EC 3.1.4.8] was selectively hydrolyzed by digestion with trypsin at the peptide bond of the single arginine residue at position 77. Selective hydrolysis was achieved by blocking the xi-amino group of Lys-41 with 2-methoxy-5-nitrotropone and subsequent digestion with trypsin in the presence of 2M urea. The trypsin-digested ribonuclease T1 was composed of two polypeptide chains containing 77 and 27 residues, though the two chains were covalently linked by a disulfide bond between Cys-6 and Cys-103. The modified enzyme lost enzymatic activity toward RNA and the ability to bind to 3'-GMP. The circular dichroic spectrum of the modified protein suggested that its conformation was extensively destroyed. It is concluded from the present results that the continuity of the peptide chain at the arginine residue is extremely important for maintaining the active conformation of the enzyme protein and for the enzymatic function of ribonuclease T1.

Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Publishing Authors By Initials

H Tamaoki,F Sakiyama,K Narita,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 79

Page Numbers: 579-89

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1976

Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Preparation and properties of trypsin-digested ribonuclease T1 split at the single arginyl peptide bond.

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Country: JAPAN

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MEDLINETA: J Biochem

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