[Preparation and preliminary identification of the monoclonal antibody to bovine bFGF]

[Preparation and preliminary identification of the monoclonal antibody to bovine bFGF] Research Abstract Details 

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[Preparation and preliminary identification of the monoclonal antibody to bovine bFGF] Abstract Text:

Shi-De Liu,Jian-Hua Zhang,Zhi-Hong Qin,Yu-Wen Yu,Miao Xing,

AIM: To prepare mAb against bovine bFGF and identify their Ig subgroups so as to establish an ELISA for detection of bFGF level. METHODS: BALB/c mice were immunized by recombinant bovine bFGF. Hybridoma cell lines which could stably secret the monoclonal antibodies to bFGF were established by cell fusion technique, and their related characteristics were identified. In addition, polyclonal antibodies to bFGF were prepared by immunization of rabbits with bovine bFGF. The mAb and polyclonal antibodies purified through protein A affinity chromatography were used to develop a sandwich ELISA for detection of bFGF level. RESULTS: Three hybridoma cell lines which could secret the mAbs IgG 1 to bFGF were obtained. The concentration of bFGF could be detected by sandwich ELISA developed with purified mAb and polyclonal antibody at nanogram level. CONCLUSION: mAb and polyclonal antibodies against to bovine bFGF have been prepared successfully, which provide powerful tool for further clinical application and related studies.

[Preparation and preliminary identification of the monoclonal antibody to bovine bFGF] Publishing Authors By Initials

SD Liu,JH Zhang,ZH Qin,YW Yu,M Xing,

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[Preparation and preliminary identification of the monoclonal antibody to bovine bFGF] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 393-4

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 27

MONTH: Jul

YEAR: 2003

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LANGUAGE: chi

NlmUniqueID: 101139110

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Grant and Affiliation Information for [Preparation and preliminary identification of the monoclonal antibody to bovine bFGF]

AFFILIATION: College of Life Science, Shenzhen University, Shenzhen 518060, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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