Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae.

Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Research Abstract Details 

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Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Abstract Text:

H Sato,Y Yamazaki,A Kodaira,H Saito,N Maehara,

Thirteen mouse monoclonal antibodies (Mabs) against the protective protein antigen (P64) of Erysipelothrix rhusiopathiae were prepared and partially characterized. The titres of the Mabs varied from 200 to 1,638,400 as determined by enzyme-linked immunosorbent assay (ELISA). Of the 13 Mabs 10, two and one belonged to the IgG2a, IgG1 and IgM subclasses, respectively. All Mabs reacted strongly with the 64 kDa protein and weakly with the 43 kDa protein upon Western blotting of the alkaline extract (AE) of E. rhusiopathiae. The protective activity (PD50/ml) of the 13 Mabs against E. rhusiopathiae infection in mice varied from < 50 to > 50,000. These Mabs were classified into three groups, highly protective Mabs, moderately protective Mabs and Mabs which did not possess protective activity, based on the protective index (ratio of the PD50/ml to the antibody titre). These results suggest that the 64 kDa protein is an effective protective antigen, which is easily cleaved into many small proteins, including the 43 kDa protein, and possesses at least two epitopes related to its protective activity and at least one epitope which is not related to protection of mice against E. rhusiopathiae infection.

Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Publishing Authors By Initials

H Sato,Y Yamazaki,A Kodaira,H Saito,N Maehara,

For similar animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred balb c research abstracts see: animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred balb c research

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Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 46

Page Numbers: 85-92

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Mar

YEAR: 1999

Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Information

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LANGUAGE: eng

NlmUniqueID: 331325

Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Keywords Mesh Terms:

KEYWORDS: Mice, Inbred BALB C

MESH TERMS: immunology

Chemical & Substance for Abstract: Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae. Information

Substance Name: SpaA protein, Erysipelothrix rhusiopathi

Registry Number: 0

Grant and Affiliation Information for Preparation and partial characterization of monoclonal antibodies against the protective protein antigen of Erysipelothrix rhusiopathiae.

AFFILIATION: Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori, Japan.

Country: GERMANY

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MEDLINETA: Zentralbl Veterinarmed B

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