Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody.

Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody. Research Abstract Details 

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Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody. Abstract Text:

Geng Kou,Jie Gao,Hao Wang,Huaiwen Chen,Bohua Li,Dapeng Zhang,Shuhui Wang,Sheng Hou,Weizhu Qian,Jianxin Dai,Yanqiang Zhong,Yajun Guo,Geng Kou,Jie Gao,Hao Wang,Huaiwen Chen,Bohua Li,Dapeng Zhang,Shuhui Wang,Sheng Hou,Weizhu Qian,Jianxin Dai,Yanqiang Zhong,Yajun Guo,Geng Kou,Jie Gao,Hao Wang,Huaiwen Chen,Bohua Li,Dapeng Zhang,Shuhui Wang,Sheng Hou,Weizhu Qian,Jianxin Dai,Yanqiang Zhong,Yajun Guo,

The purpose of this study was to develop paclitaxel-loaded poly(lactide-co-glycolide) (PLGA) nanoparticles coated with cationic SM5-1 single-chain antibody (scFv) containing a polylysine (SMFv-polylys). SM5-1 scFv (SMFv) is derived from SM5-1 monoclonal antibody, which binds to a 230 kDa membrane protein specifically expressed on melanoma, hepatocellular carcinoma and breast cancer cells. SMFv-polylys was expressed in Escherichia coli and purified by cation-exchange chromatography. Purified SMFv-polylys was fixed to paclitaxel-loaded PLGA nanoparticles to form paclitaxel-loaded PLGA nanoparticles coated with SMFv-polylys (Ptx-NP-S). Ptx-NP-S was shown to retain the specific antigen-binding affinity of SMFv-polylys to SM5-1 binding protein-positive Ch-hep-3 cells. Finally, the cytotoxicity of Ptx-NP-S was evaluated by a non-radioactive cell proliferation assay. It was demonstrated that Ptx-NP-S had significantly enhanced in vitro cytotoxicity against Ch-hep-3 cells as compared with non-targeted paclitaxel-loaded PLGA nanoparticles. In conclusion, our results suggest that cationic SMFv-polylys has been successfully generated and may be used as targeted ligand for preparing cancer-targeted nanoparticles.

Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody. Publishing Authors By Initials

G Kou,J Gao,H Wang,H Chen,B Li,D Zhang,S Wang,S Hou,W Qian,J Dai,Y Zhong,Y Guo,G Kou,J Gao,H Wang,H Chen,B Li,D Zhang,S Wang,S Hou,W Qian,J Dai,Y Zhong,Y Guo,G Kou,J Gao,H Wang,H Chen,B Li,D Zhang,S Wang,S Hou,W Qian,J Dai,Y Zhong,Y Guo,

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Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry and molecular biology

VOLUME: 40

Page Numbers: 731-9

Journal Abbreviation: J. Biochem. Mol. Biol.

ISSN: 1225-8687

DAY: 30

MONTH: Sep

YEAR: 2007

Preparation and Characterization of Paclitaxel-loaded PLGA nanoparticles coated with cationic SM5-1 single-chain antibody. Information

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LANGUAGE: eng

NlmUniqueID: 9702084

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AFFILIATION: International Joint Cancer Institute and College of Pharmacy, Second Military Medical University, New Library Building West 10th-11th Floor, 800 Xiang Yin Road, Shanghai 200433, People's Republic of China.

Country: Korea (South)

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MEDLINETA: J Biochem Mol Biol

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