[Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor]

[Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor] Research Abstract Details 

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[Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor] Abstract Text:

Ying Liu,Xue-Song Liu,Yong Zhu,Li-Hua Chen,Yun-Xin Cao,Xiao-Guang Xu,Yi Hu,Ying Tian,Bo-Quan Jin,

AIM:To prepare monoclonal antibodies against human TACI and study the distribution of TACI on peripheral blood nuclear cells(PBMCs). METHODS: Murine mAbs were prepared by hybridoma technique. The distribution of TACI on PBMCs were identified by immunofluorescence staining, FCM analysis as well as immunohistochemical staining. RESULTS: Two hybridoma cell lines secreting mAbs against TACI were obtained. The titers of ascitic mAbs was 10(-7) and their Ig subclasses were IgG1. Both could recognize natural TACI on T cells. In the PHA activated models, TACI was expressed mainly on activated CD4(+) T cells. CONCLUSION: Two mAbs can recognize natural TACI, which will be of greater value in research on the structure and functions of TACI.

[Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor] Publishing Authors By Initials

Y Liu,XS Liu,Y Zhu,LH Chen,YX Cao,XG Xu,Y Hu,Y Tian,BQ Jin,

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[Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 168-9

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 20

MONTH: Mar

YEAR: 2003

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LANGUAGE: chi

NlmUniqueID: 101139110

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Grant and Affiliation Information for [Preparation and characterization of monoclonal antibodies against human transmembrane activator and calcium modulator and cyclophilin ligand interactor]

AFFILIATION: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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