Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations.

Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Research Abstract Details 

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Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Abstract Text:

Elisabeth Mansfield,Eric E Ross,Craig A Aspinwall,

Analysis of protein and peptide mixtures via capillary electrophoresis is hindered by nonspecific adsorption of analytes to the capillary walls, resulting in poor separations and quantitative reproducibility. Phospholipid bilayer (PLB) coatings are very promising for improving protein and peptide separations due to the native resistance to nonspecific protein adsorption offered by PLBs; however, these coatings display limited chemical and temporal stability. Here, we show the preparation and characterization of a highly cross-linked, polymerized phospholipid capillary coating prepared using bis-SorbPC. Poly(bis-SorbPC) PLB coatings are prepared in situ within fully enclosed fused silica capillaries via self-assembly and radical polymerization. Polymerization of the PLB coating stabilizes the membrane against desorption from the surface and migration in an electric field, improves the temporal and chemical stability, and allows for the separation of both cationic and anionic proteins, while preserving the native resistance to nonspecific protein adsorption of natural PLBs.

Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Publishing Authors By Initials

E Mansfield,EE Ross,CA Aspinwall,

For similar proteins research abstracts see: proteins research

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Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical chemistry

VOLUME: 79

Page Numbers: 3135-41

Journal Abbreviation:

ISSN: 0003-2700

DAY: 21

MONTH: 03

YEAR: 2007

Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370536

Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations. Information

Substance Name: 1,2-bis(10(2',4'-hexadienoyloxy)decanoyl

Registry Number: 124154-20-7

Grant and Affiliation Information for Preparation and characterization of cross-linked phospholipid bilayer capillary coatings for protein separations.

AFFILIATION: Department of Chemistry, University of Arizona, Tucson, Arizona 85721, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM74522

ACRONYM: GM

MEDLINETA: Anal Chem

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