[Preparation and characterization of a McAb against tissue factor pathway inhibitor]

[Preparation and characterization of a McAb against tissue factor pathway inhibitor] Research Abstract Details 

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[Preparation and characterization of a McAb against tissue factor pathway inhibitor] Abstract Text:

Y Yang,X He,J Li,S He,

OBJECTIVE: To prepare monoclonal antibody (McAb) against tissue factor pathway inhibitor (TFPI). METHODS: Twice intrasplenic immunization of BALB/c mice with TFPI-binded nitrocellulose paper followed by an intraperitoneal boost with TFPI raised two hybridoma cell lines, 4F8 and 4F4, by hybridoma technique, 4F8 was studied in detail. The mode value of its chromosome is 93. The Ig class of McAb 4F8 was IgG1. The McAb was purified from ascites by a simple procedure of caprylic acid pre cipitation. The purified McAb was conjugated with horseradish peroxidase (HRP) by a modified periodate oxidation procedure. RESULTS: Immunoblotting indicated that the McAb recognized a single band of TFPI with a molecular weight of 34800. The McAb was also proved to shorten markedly the dilute thromboplastin coagulation time of factor IX-deficient and normal plasma. The results of Sandwich ELISA using the HRP labeled McAb showed that the mean TFPI in normal human plasma is 103.2 +/- 11.5 microg/L, being similar to that measured with TFPI ELISA kit from America Diagnostica Inc. (98.4 +/- 10.3 microg/L) (r=0.92). CONCLUSION: McAb 4F8 may be a useful reagent for TFPI research.

[Preparation and characterization of a McAb against tissue factor pathway inhibitor] Publishing Authors By Initials

Y Yang,X He,J Li,S He,

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[Preparation and characterization of a McAb against tissue factor pathway inhibitor] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 18

Page Numbers: 118-22

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 29

MONTH: Mar

YEAR: 1997

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LANGUAGE: chi

NlmUniqueID: 8212398

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Grant and Affiliation Information for [Preparation and characterization of a McAb against tissue factor pathway inhibitor]

AFFILIATION: Department of Physiology, Hunan Medical University, Changsha 410078.

Country: China

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MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

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