[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2]

[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2] Research Abstract Details 

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[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2] Abstract Text:

Jun-e Li,Guan-lin Sun,Ying-li Wu,Wei-li Wu,

OBJECTIVE: To investigate the apoptotic inducing effect of As(2)S(2) on K562 cells. METHODS: The apoptotic inducing effect of As(2)S(2) on K562 cells was determined by flow cytometry, DNA fragmentation analysis and morphology observation. Expression of protein was determined by Western-blot. RT-PCR was used to evaluate changes in gene expression. RESULTS: Apoptosis of K562 cells was induced by 48 - 72 h exposure to 5 micromol/L As(2)S(2). Apoptosis was induced in (34.4 +/- 3.3)% treated cells by 72 h exposure to 3 micro mol/L As(2)S(2), in (21.8 +/- 3.6)% treated cells by 48 h exposure to 5 micromol/L As(2)S(2) and in (46.0 +/- 5.2)% treated cells by 72 h exposure to As(2)S(2) at the same concentration. With 5 micromol/L As(2)S(2), the protein level of Bcr-Abl and JAK2 decreased, while bax expression was upregulated and c-myc was downregulated both in protein and mRNA level. The activity of caspase 3 in K562 cells was increased by As(2)S(2). As(2)S(2) also induced apoptosis of fresh mononuclear cells derived from chronic myelogenous leukemia (CML) patients. CONCLUSION: As(2)S(2) can induce apoptosis of CML cells. The decline of Bcr-Abl may play an important role. The upregulation of bax, increase of the activity of caspase 3, downregulation of c-myc and decrease of JAK2 may also be involved in the mechanism.

[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2] Publishing Authors By Initials

JE Li,GL Sun,YL Wu,WL Wu,

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[Preliminary study on the molecular mechanism of K562 cell apoptosis induced by As2S2] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua zhong liu za zhi [Chinese journal of onco

VOLUME: 25

Page Numbers: 220-4

Journal Abbreviation: Zhonghua Zhong Liu Za Zhi

ISSN: 0253-3766

DAY: 3

MONTH: May

YEAR: 2003

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LANGUAGE: chi

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AFFILIATION: Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China.

Country: China

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MEDLINETA: Zhonghua Zhong Liu Za Zhi

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