Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments.

Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments. Research Abstract Details 

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Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments. Abstract Text:

PURPOSE: Fluorescent polymerase chain reaction (PCR) is a multipurpose technique that can be used for diagnosing sex, single-gene defects, and trisomies as well as determining DNA fingerprints from single cells. However, its effectiveness must be assessed before clinical preimplantation genetic diagnosis (PGD) application. METHODS: Single and multiplex fluorescent PCR was applied to single cells and blastomeres. RESULTS: Fluorescent PCR can be used to diagnose sex from blastomeres and has been successfully applied in a clinical PGD sexing program resulting in a confirmed pregnancy. A further major advantage of fluorescent PCR is the ability to multiplex, providing multiple diagnoses and DNA fingerprints with a high reliability (approximately 75% for trisomy, 86% for DNA fingerprint) and good accuracy (70-80%). Allele dropout in multiplex PCR is approximately 20% per allele and does not appear to be associated with the fragment size. CONCLUSIONS: Fluorescent PCR is a powerful technique for PGD, and the effects of allele dropout must be considered, particularly in multiplex PCR.

Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments. Publishing Authors By Initials

For similar genetic processes: sex determination (genetics) research abstracts see: genetic processes: sex determination (genetics) research

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Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 16

Page Numbers: 199-206

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Apr

YEAR: 1999

Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments. Keywords Mesh Terms:

KEYWORDS: Sex Determination (Genetics)

MESH TERMS: trends

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Grant and Affiliation Information for Preimplantation genetic diagnosis using fluorescent polymerase chain reaction: results and future developments.

AFFILIATION: Institute of Pathology, Algernon Firth Building, Leeds University, UK.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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