Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore.

Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Research Abstract Details 

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Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Abstract Text:

Kenneth H S Wong,G C Ng,Raymond T P Lin,H Yoshikawa,Mark B Taylor,Kevin S W Tan,

Blastocystis is an enteric protozoan parasite commonly found in humans and animals. Phylogenetic and genotypic analyses have shown that Blastocystis exhibits extreme genetic diversity, and humans are host to a number of zoonotic isolates. In the present study, the prevalence of Blastocystis in 276 stool samples from a hospital in Singapore was examined, and for the first time, riboprinting using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to determine the genetic diversity of the Blastocystis isolated from the Singapore population. The prevalence rate was determined to be 3.3% (9/276), and Blastocystis displaying two main ribotypes were isolated. As a comparison, we performed PCR-RFLP using two different published methodologies, and both methods allowed the isolates to be divided into two distinct groups based on their riboprint patterns. According to a recently proposed classification scheme, 78% (7/9) of the isolates were of subtype 3, while 22% (2/9) were subtype 1. The predominance of subtype 3 in an urbanized city state such as Singapore is in agreement with the idea that subtype 3 is a genotype of human origin.

Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Publishing Authors By Initials

KH Wong,GC Ng,RT Lin,H Yoshikawa,MB Taylor,KS Tan,

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Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Parasitology research

VOLUME: 102

Page Numbers: 663-70

Journal Abbreviation: Parasitol. Res.

ISSN: 0932-0113

DAY: 7

MONTH: 12

YEAR: 2007

Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore.

AFFILIATION: Laboratory of Molecular and Cellular Parasitology, National University of Singapore, Singapore 5 Science Drive 2, Singapore, 117597, Singapore.

Country: Germany

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MEDLINETA: Parasitol Res

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