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Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test.

Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test. Research Abstract Details 

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  • Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test. Abstract Text:

    m d eaglesomeM D Eaglesome,s a millerS A Miller,

    The ability of liposome-treated fresh and frozen spermatozoa from two bulls to interact with zona-free hamster oocytes was examined to show whether the in vitro test results would correspond with in vivo fertility as indicated by the 60 to 90 d nonreturn to service rates which, using frozen semen, were 77 and 59%, respectively. The motility of spermatozoa in washed suspensions was also rated. Hamster test results were obtained using three ejaculates from each bull both as fresh and frozen semen. The results with frozen semen corresponded with fertility. The averages of three hamster tests for oocyte penetration rates and mean number of spermatozoa per penetrated oocyte comparing spermatozoa from the bull with the higher fertility with spermatozoa from the bull with the lower fertility were 91% and 2.7 versus 56% and 1.4, respectively. Spermatozoa washed from frozen semen from the bull with the higher fertility interacted with hamster oocytes at the higher rate even when sperm motility was rated the same for both bulls. By contrast, fresh spermatozoa from the lower fertility bull interacted with hamster oocytes at a higher rate than spermatozoa from the higher fertility bull in six tests, comparing six ejaculates of fresh semen from both bulls. Comparing the higher fertility bull with the lower fertility bull, the average of six tests for oocyte penetration rates and mean number of spermatozoa per penetrated oocyte were 60% and 1.6 versus 89% and 3.0, respectively. This suggests that this hamster test cannot be used with fresh semen to predict relative levels of fertility of frozen semen. Also, the subjective rating of sperm motility did not correspond with the in vitro oocyte penetrating ability of the spermatozoa.

    Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test. Publishing Authors By Initials

    md eaglesomeMD Eaglesome,sa millerSA Miller,

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    Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Theriogenology

    VOLUME: 31

    Page Numbers: 643-51

    Journal Abbreviation: Theriogenology

    ISSN: 0093-691X

    DAY: 26

    MONTH: Mar

    YEAR: 1989

    Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test. Information

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    LANGUAGE: eng

    NlmUniqueID: 421510

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    Grant and Affiliation Information for Prediction of fertility of bovine semen: Preliminary studies with the hamster egg penetration test.

    AFFILIATION: Agriculture Canada, Animal Diseases Research Institute, NEPEAN P.O. Box 11300, Station H, Nepean, Ontario, Canada K2H 8P9.

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: Theriogenology

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