Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates.

Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates. Research Abstract Details 

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Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates. Abstract Text:

S Pichyangkul,U Kum-Arb,K Yongvanitchit,A Limsalakpetch,M Gettayacamin,D E Lanar,L A Ware,V A Stewart,D G Heppner,P Mettens,J D Cohen,W R Ballou,M M Fukuda,S Pichyangkul,U Kum-Arb,K Yongvanitchit,A Limsalakpetch,M Gettayacamin,D E Lanar,L A Ware,V A Stewart,D G Heppner,P Mettens,J D Cohen,W R Ballou,M M Fukuda,S Pichyangkul,U Kum-Arb,K Yongvanitchit,A Limsalakpetch,M Gettayacamin,D E Lanar,L A Ware,V A Stewart,D G Heppner,P Mettens,J D Cohen,W R Ballou,M M Fukuda,

Several lines of evidence suggest that targeting pre-erythrocytic-stage parasites for malaria vaccine development can provide sterile immunity. The objectives of this study were (i) to evaluate preclinically the safety and immunogenicity of a new recombinant pre-erythrocytic-stage antigen, liver-stage antigen 1 (LSA1), in nonhuman primates; and (ii) to investigate the potential for immune interference between LSA1 and the leading malaria vaccine candidate, RTS,S, by comparing the immune responses after single-antigen vaccination to responses after simultaneous administration of both antigens at separate sites. Using a rhesus monkey model, we found that LSA1 formulated with the GlaxoSmithKline proprietary adjuvant system AS01B (LSA1/AS01B) was safe and immunogenic, inducing high titers of antigen-specific antibody and CD4+ T-cell responses, as monitored by the production of interleukin-2 and gamma interferon, using intracellular cytokine staining. RTS,S/AS01B vaccination was well tolerated and demonstrated robust antibody and moderate CD4+ T-cell responses to circumsporozoite protein (CSP) and HBsAg. Positive CD8+ T-cell responses to HBsAg were detected, whereas the responses to CSP and LSA1 were negligible. For both LSA1/AS01B and RTS,S/AS01B, no statistically significant differences were observed between individual and concurrent administration in the magnitude or duration of antibody and T-cell responses. Our results revealed that both pre-erythrocytic-stage antigens were safe and immunogenic, administered either separately or simultaneously to rhesus monkeys, and that no significant immune cross interference occurred with concurrent separate-site administration. The comparison of the profiles of immune responses induced by separate-site and single-site vaccinations with LSA1 and RTS,S warrants further investigation.

Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates. Publishing Authors By Initials

S Pichyangkul,U Kum-Arb,K Yongvanitchit,A Limsalakpetch,M Gettayacamin,DE Lanar,LA Ware,VA Stewart,DG Heppner,P Mettens,JD Cohen,WR Ballou,MM Fukuda,S Pichyangkul,U Kum-Arb,K Yongvanitchit,A Limsalakpetch,M Gettayacamin,DE Lanar,LA Ware,VA Stewart,DG Heppner,P Mettens,JD Cohen,WR Ballou,MM Fukuda,S Pichyangkul,U Kum-Arb,K Yongvanitchit,A Limsalakpetch,M Gettayacamin,DE Lanar,LA Ware,VA Stewart,DG Heppner,P Mettens,JD Cohen,WR Ballou,MM Fukuda,

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Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Infection and immunity

VOLUME: 76

Page Numbers: 229-38

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ISSN: 1098-5522

DAY: 22

MONTH: 10

YEAR: 2007

Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates. Information

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LANGUAGE: eng

NlmUniqueID: 246127

Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates. Keywords Mesh Terms:

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Grant and Affiliation Information for Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates.

AFFILIATION: Department of Immunology and Medicine, USAMC-AFRIMS, 315/6 Rajvithi Rd., Bangkok 10400, Thailand. Sathitp@afrims.org

Country: United States

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MEDLINETA: Infect Immun

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Preclinical evaluation of the safety and immunogenicity of a vaccine consisting of Plasmodium falciparum liver-stage antigen 1 with adjuvant AS01B administered alone or concurrently with the RTS,S/AS01B vaccine in rhesus primates Related Publications

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