Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane.

Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Research Abstract Details 

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Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Abstract Text:

M Nakai,T Hase,H Matsubara,

A major 70 kDa protein of the yeast mitochondrial outer membrane is coded by a nuclear gene, synthesized on cytoplasmic ribosomes, and transported to the mitochondrial outer membrane. In order to investigate in detail the information necessary for localizing the 70 kDa protein at the outer membrane, we have examined the intracellular and intramitochondrial location of fusion proteins which consist of various lengths of the amino-terminal region of the 70 kDa protein with an enzymatically active beta-galactosidase. The results indicate that the extreme amino-terminal 12 amino acids of the 70 kDa protein function as a targeting sequence, whereas the subsequent uncharged region (up to residue 29) is necessary for "stop-transfer" and "anchoring" functions. Moreover, we have found that a fusion protein which contained the amino-terminal 19 amino acids of the 70 kDa protein is localized on the outer membrane as well as in the matrix space. Changes in the dual localization of this fusion protein accompanied its overproduction or expression in a respiration-deficient yeast mutant.

Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Publishing Authors By Initials

M Nakai,T Hase,H Matsubara,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: galactosidases: beta-galactosidase research

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Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 105

Page Numbers: 513-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1989

Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Precise determination of the mitochondrial import signal contained in a 70 kDa protein of yeast mitochondrial outer membrane.

AFFILIATION: Department of Biology, Faculty of Science, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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