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Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia.

Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia. Research Abstract Details 

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  • Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia. Abstract Text:

    haile f yancyHaile F Yancy,tyler s zemlakTyler S Zemlak,jacquline a masonJacquline A Mason,jewell d washingtonJewell D Washington,bradley j tengeBradley J Tenge,ngoc-lan t nguyenNgoc-Lan T Nguyen,james d barnettJames D Barnett,warren e savaryWarren E Savary,walter e hillWalter E Hill,michelle m mooreMichelle M Moore,frederick s fryFrederick S Fry,spring c randolphSpring C Randolph,patricia l rogersPatricia L Rogers,paul d n hebertPaul D N Hebert,

    The use of a DNA-based identification system (DNA barcoding) founded on the mitochondrial gene cytochrome c oxidase subunit I (COI) was investigated for updating the U.S. Food and Drug Administration Regulatory Fish Encyclopedia (RFE; http://www.cfsan.fda.gov/-frf/rfe0.html). The RFE is a compilation of data used to identify fish species. It was compiled to help regulators identify species substitution that could result in potential adverse health consequences or could be a source of economic fraud. For each of many aquatic species commonly sold in the United States, the RFE includes high-resolution photographs of whole fish and their marketed product forms and species-specific biochemical patterns for authenticated fish species. These patterns currently include data from isoelectric focusing studies. In this article, we describe the generation of DNA barcodes for 172 individual authenticated fish representing 72 species from 27 families contained in the RFE. These barcode sequences can be used as an additional identification resource. In a blind study, 60 unknown fish muscle samples were barcoded, and the results were compared with the RFE barcode reference library. All 60 samples were correctly identified to species based on the barcoding data. Our study indicates that DNA barcoding can be a powerful tool for species identification and has broad potential applications.

    Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia. Publishing Authors By Initials

    hf yancyHF Yancy,ts zemlakTS Zemlak,ja masonJA Mason,jd washingtonJD Washington,bj tengeBJ Tenge,nl nguyenNL Nguyen,jd barnettJD Barnett,we savaryWE Savary,we hillWE Hill,mm mooreMM Moore,fs fryFS Fry,sc randolphSC Randolph,pl rogersPL Rogers,pd hebertPD Hebert,

    For similar abstracts research abstracts see: abstracts research

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    Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of food protection

    VOLUME: 71

    Page Numbers: 210-7

    Journal Abbreviation: J. Food Prot.

    ISSN: 0362-028X

    DAY: 1

    MONTH: Jan

    YEAR: 2008

    Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia. Information

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    LANGUAGE: eng

    NlmUniqueID: 7703944

    Potential use of DNA barcodes in regulatory science: applications of the Regulatory Fish Encyclopedia. Keywords Mesh Terms:

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    AFFILIATION: U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, 8401 Muirkirk Road, Laurel, Maryland 20708, USA. haile.yancy@fda.hhs.gov

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: J Food Prot

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