Potential microbial risk factors related to soil amendments and irrigation water of potato crops.

Potential microbial risk factors related to soil amendments and irrigation water of potato crops. Research Abstract Details 

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Potential microbial risk factors related to soil amendments and irrigation water of potato crops. Abstract Text:

M V Selma,A Allende,F ,P ,R Aznar,M I Gil,M V Selma,A Allende,F ,P ,R Aznar,M I Gil,

Aims: This study assesses the potential microbial risk factors related to the use of soil amendments and irrigation water on potato crops, cultivated in one traditional and two intensive farms during two harvest seasons. Methods and Results: The natural microbiota and potentially pathogenic micro-organisms were evaluated in the soil amendment, irrigation water, soil and produce. Uncomposted amendments and residual and creek water samples showed the highest microbial counts. The microbial load of potatoes harvested in spring was similar among the tested farms despite the diverse microbial levels of Listeria spp. and faecal coliforms in the potential risk sources. However, differences in total coliform load of potato were found between farms cultivated in the autumn. Immunochromatographic rapid tests and the BAM's reference method (Bacteriological Analytical Manual; AOAC International) were used to detect Escherichia coli O157:H7 from the potential risk sources and produce. Confirmation of the positive results by polymerase chain reaction procedures showed that the immunochromatographic assay was not reliable as it led to false-positive results. Conclusions: The potentially pathogenic micro-organisms of soil amendment, irrigation water and soil samples changed with the harvest seasons and the use of different agricultural practices. However, the microbial load of the produce was not always influenced by these risk sources. Improvements in environmental sample preparation are needed to avoid interferences in the use of immunochromatographic rapid tests. Significance and Impact of the Study: The potential microbial risk sources of fresh produce should be regularly controlled using reliable detection methods to guarantee their microbial safety.

Potential microbial risk factors related to soil amendments and irrigation water of potato crops. Publishing Authors By Initials

MV Selma,A Allende,F ,P ,R Aznar,MI Gil,MV Selma,A Allende,F ,P ,R Aznar,MI Gil,

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Potential microbial risk factors related to soil amendments and irrigation water of potato crops. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of applied microbiology

VOLUME: 103

Page Numbers: 2542-9

Journal Abbreviation: J. Appl. Microbiol.

ISSN: 1364-5072

DAY: 29

MONTH: Dec

YEAR: 2007

Potential microbial risk factors related to soil amendments and irrigation water of potato crops. Information

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LANGUAGE: eng

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AFFILIATION: Research group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS-CSIC, Espinardo, Murcia, Spain, and Department of Microbiology and Ecology, University of Valencia, Burjassot, Valencia, Spain.

Country: England

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MEDLINETA: J Appl Microbiol

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