Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles.

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Abstract Text:

Xue-Qing Zhang,Christopher E Dahle,Nicki K Baman,Nathan Rich,George J Weiner,Aliasger K Salem,

CpG ODN stimulates a TH1 response through its receptor Toll-like receptor 9 (TLR9). TLR9 is a receptor that is found intracellularly. Microparticles are efficiently internalized by dendritic cells (DCs) and macrophages and would thus be an ideal delivery vehicle for CpG ODN to reach its target site thereby enhancing the TH1 response to an antigen also encapsulated in the microparticle. Here, we show that careful control over fabrication parameters can produce biodegradable microparticles with predictable size distributions, surface morphology, and shape. Entrapment efficiencies of the model antigen OVA ranged from 19% to 23% with an average loading of 10 microg/mg of microparticles. For CpG ODN, these values were 33% to 35%, which corresponded to an average loading of 8.5 microg/mg of microparticles. The microparticles release CpG ODN and OVA in a burst followed by sustained release profile. At the highest concentration of microparticles incubated with a pure DC cell line, 92% of DCs had internalized microparticles by 16 hours, confirming that DCs efficiently take up the microparticles. Microparticles are capable of inducing DC maturation as determined by up-regulation of CD80 and CD86 markers. Although the presence of CpG ODN in the microparticles did not impact on the phenotype of the DCs, it was necessary for DCs to induce activation of antigen-specific T cells as indicated by interferon-gamma production. Microparticles entrapping both antigen and CpG ODN induced significantly higher amounts of anti-OVA antibody production than other preparations such as the soluble OVA and CpG ODN (P<0.01) and stimulated stronger IgG2a production than delivery of microparticles entrapping antigen alone. We conclude that co-encapsulating immunostimulatory CpG ODN and antigen in degradable microparticles is an effective approach to enhancing development of a TH1 immune response.

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Publishing Authors By Initials

XQ Zhang,CE Dahle,NK Baman,N Rich,GJ Weiner,AK Salem,

For similar therapeutics: biological therapy: immunotherapy: immunization: immunotherapy, active: vaccination research abstracts see: therapeutics: biological therapy: immunotherapy: immunization: immunotherapy, active: vaccination research

PUBMED ID PMID:

MEDLINE DATE:

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of immunotherapy (Hagerstown, Md. : 1997)

VOLUME: 30

Page Numbers: 469-78

Journal Abbreviation: J. Immunother.

ISSN: 1524-9557

DAY: 3

MONTH: 12

YEAR: 2007

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9706083

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Keywords Mesh Terms:

KEYWORDS: Vaccination

MESH TERMS: immunology

Chemical & Substance for Abstract: Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles.

AFFILIATION: Division of Pharmaceutics, College of Pharmacy, University of Iowa, 115 S. Grand Avenue, Iowa City, IA, USA.

Country: United States

AGENCY: United States NCI

GRANT: P50 CA 097274-04

ACRONYM: CA

MEDLINETA: J Immunother (1997)

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles Related Publications

• A comparative study of the antigen-specific immune response induced by co-delivery of CpG ODN and antigen using fusion molecules or biodegradable microparticles.
• Abnormal electrophoretic pattern of glycosylated hemoglobin in sickle cell disorders.
• An n-3 fatty acid deficient diet affects mouse spatial learning in the Barnes circular maze.
• Artificial rearing with docosahexaenoic acid and n-6 docosapentaenoic acid alters rat tissue fatty acid composition.
• Bilateral differences in the net joint torques during the squat exercise.
• Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery.
• Correlations between thoracic kyphosis and scapulohumeral motion: 1625: board #115 may 30 2:00 PM - 3:30 PM.
• Emerging approaches in hepatocellular carcinoma.
• Evaluation of intravesical potassium sensitivity test and bladder biopsy in patients with chronic prostatitis/chronic pelvic pain syndrome.
• Fiberoptic endoscopic evaluation of swallowing in assessing aspiration after transhiatal esophagectomy.
• Hypertension, urbanization, social and spatial disparities: a cross-sectional population-based survey in a West African urban environment (Ouagadougou, Burkina Faso).
• Integrated CMOS Electronic System-on-Chip for Medical sensors and Actuators.
• Management of acute diverticulitis in the East anglian region: result of a United kingdom regional survey.
• Maternal smoking is associated with decreased 5-methyltetrahydrofolate in cord plasma.
• Mechanics of a unilateral squat exercise to different depths: implications for rehabilitation: 1621: board #111 may 30 2:00 PM - 3:30 PM.
• Multifunctional nanorods for biomedical applications.
• Osteoid osteoma; report of a case with probable double lesion.
• Potent antigen-specific immune responses stimulated by codelivery of CpG ODN and antigens in degradable microparticles.
• Potential attenuation of disease progression in recurrent prostate cancer with plant-based diet and stress reduction.
• Radiation dose limits and liver toxicities resulting from multiple yttrium-90 radioembolization treatments for hepatocellular carcinoma.
• Radioembolization with 90Y microspheres: angiographic and technical considerations.
• Radioembolization with 90Y microspheres: technical considerations.
• Reduction of arteriohepatovenous shunting by temporary balloon occlusion in patients undergoing radioembolization.
• Regional hemodynamics and oxygen supply during isovolemic hemodilution in the absence and presence of high-grade beta-adrenergic blockade.
• Role of liver and plasma lipoproteins in selective transport of n-3 fatty acids to tissues: a comparative study of 14C-DHA and 3H-oleic acid tracers.
• Sensing and Feedback Stimulation via The Wireless ZigBee Protocol.
• Spatial control over cell attachment by partial solvent entrapment of poly lysine in microfluidic channels.
• Weightlifting Exercise Potentiates Joint Mechanical Work during Vertical Jumping: 1805: Board #93 May 31 9:00 AM 10:30 AM.
• Whole body distribution of deuterated linoleic and {alpha}-linolenic acids and their metabolites in the rat.
• [Not Available.]