Post-translational modifications in the nuclear region of young, aged, and cataract human lenses.

Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Research Abstract Details 

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Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Abstract Text:

Peter G Hains,Roger J W Truscott,Peter G Hains,Roger J W Truscott,

The urea-soluble proteins from the nucleus of two young, two aged, and two early-stage nuclear cataract lenses were subjected to tryptic digestion and analysis by 2D LC-MS/MS. Several novel post-translational modifications were identified. Deamidation was, by far, the most common modification. A number of differences were found in cataract compared to normal lenses, most notably an increase in the number of oxidized tryptophan residues. Semiquantitative analysis revealed that there appeared to be a trend toward increased levels of deamidation with age; however, there was no apparent increase upon the onset of nuclear cataract. This is in contrast to Trp oxidation, where an increase in the extent of modification was apparent in cataract lenses when compared to aged normal lenses. These findings suggest Trp oxidation may be involved in nuclear cataract development.

Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Publishing Authors By Initials

PG Hains,RJ Truscott,PG Hains,RJ Truscott,

For similar investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research abstracts see: investigative techniques: chemistry, analytical: mass spectrometry: tandem mass spectrometry research

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Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of proteome research

VOLUME: 6

Page Numbers: 3935-43

Journal Abbreviation: J. Proteome Res.

ISSN: 1535-3893

DAY: 7

MONTH: 09

YEAR: 2007

Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101128775

Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Keywords Mesh Terms:

KEYWORDS: Tandem Mass Spectrometry

MESH TERMS: metabolism

Chemical & Substance for Abstract: Post-translational modifications in the nuclear region of young, aged, and cataract human lenses. Information

Substance Name: Crystallins

Registry Number: 0

Grant and Affiliation Information for Post-translational modifications in the nuclear region of young, aged, and cataract human lenses.

AFFILIATION: Save Sight Institute, University of Sydney, Sydney, NSW, 2001, Australia.

Country: United States

AGENCY: United States NEI

GRANT: R01EY013570-03

ACRONYM: EY

MEDLINETA: J Proteome Res

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