Positive and negative regulation of Tetrahymena telomerase holoenzyme.

Positive and negative regulation of Tetrahymena telomerase holoenzyme. Research Abstract Details 

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Positive and negative regulation of Tetrahymena telomerase holoenzyme. Abstract Text:

Keren L Witkin,Ramadevi Prathapam,Kathleen Collins,

Telomerase replenishes the telomeric repeats that cap eukaryotic chromosome ends. To perform DNA synthesis, the active site of telomerase reverse transcriptase (TERT) copies a template within the integral telomerase RNA (TER). In vivo, TERT and TER and additional subunits form a telomerase holoenzyme capable of telomere elongation. We previously purified epitope-tagged Tetrahymena thermophila TERT and characterized two of the associated proteins. Here we characterize the remaining two proteins that were enriched by TERT purification. The primary sequence of the p75 polypeptide lacks evident homology with other proteins, whereas the p20 polypeptide is the Tetrahymena ortholog of a conserved multifunctional protein, Skp1. Genetic depletion of p75 induced telomere shortening without affecting the accumulation of TER or TERT, suggesting that p75 promotes telomerase function at the telomere. Affinity purification of p75 coenriched telomerase activity and each other known telomerase holoenzyme protein. On the other hand, genetic depletion of Skp1p induced telomere elongation, suggesting that this protein plays a negative regulatory role in the maintenance of telomere length homeostasis. Affinity purification of Skp1p did not detectably enrich active telomerase but did copurify ubiquitin ligase machinery. These studies reveal additional complexity in the positive and negative regulation of Tetrahymena telomerase function.

Positive and negative regulation of Tetrahymena telomerase holoenzyme. Publishing Authors By Initials

KL Witkin,R Prathapam,K Collins,

For similar animals: invertebrates: protozoa: ciliophora: oligohymenophorea: hymenostomatida: tetrahymenina: tetrahymena research abstracts see: animals: invertebrates: protozoa: ciliophora: oligohymenophorea: hymenostomatida: tetrahymenina: tetrahymena research

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Positive and negative regulation of Tetrahymena telomerase holoenzyme. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular and cellular biology

VOLUME: 27

Page Numbers: 2074-83

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 0270-7306

DAY: 12

MONTH: 01

YEAR: 2007

Positive and negative regulation of Tetrahymena telomerase holoenzyme. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

Positive and negative regulation of Tetrahymena telomerase holoenzyme. Keywords Mesh Terms:

KEYWORDS: Tetrahymena

MESH TERMS: genetics

Chemical & Substance for Abstract: Positive and negative regulation of Tetrahymena telomerase holoenzyme. Information

Substance Name: Telomerase

Registry Number: EC 2.7.7.49

Grant and Affiliation Information for Positive and negative regulation of Tetrahymena telomerase holoenzyme.

AFFILIATION: Department of Molecular and Cell Biology, University of California at Berkeley, CA 94720-3204, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM54198

ACRONYM: GM

MEDLINETA: Mol Cell Biol

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