Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes.

Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Research Abstract Details 

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Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Abstract Text:

K Morohashi,H Sadano,Y Okada,T Omura,

The hydroxylation of n-hexane by rat liver microsomes was studied and the contribution of different molecular species of cytochrome P-450 to the hydroxylation reaction was examined. In the case of untreated rats, the products of NADPH-dependent n-hexane hydroxylation were 1-, 2-, and 3-hexanols, and the major one was 2-hexanol. Phenobarbital (PB) treatment of animals resulted in a significant increase of the hydroxylation activity. The formation of 2- and 3-hexanols was much more significantly increased than that of 1-hexanol. On the other hand, 3-methylcholanthrene (MC) treatment stimulated the formation of 3-hexanol and the formation of the other two isomeric alcohols was rather decreased. These observations suggested the position specificities of the PB-inducible form (P-450(PB)) and MC-inducible form (P-450(MC)) of cytochrome P-450 in the hydroxylation of n-hexane. Inhibition experiments using antibodies specific to P-450(PB) and P-450(MC) also indicated that P-450(PB) was more active in the hydroxylation at the 2-position whereas P-450(MC) was more specific for the 3-position. NADPH-dependent n-hexane hydroxylation systems were reconstituted by the use of purified NADPH-cytochrome P-450 reductase and P-450(PB) or P-450(MC), and the activities of the reconstituted systems supported the proposed position specificities of these two forms of cytochrome P-450 in n-hexane hydroxylation.

Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Publishing Authors By Initials

K Morohashi,H Sadano,Y Okada,T Omura,

For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

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Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 413-9

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1983

Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Keywords Mesh Terms:

KEYWORDS: Rats, Inbred Strains

MESH TERMS: metabolism

Chemical & Substance for Abstract: Position specificity in n-hexane hydroxylation by two forms of cytochrome P-450 in rat liver microsomes. Information

Substance Name: NADPH-Ferrihemoprotein Reductase

Registry Number: EC 1.6.2.4

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Country: JAPAN

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MEDLINETA: J Biochem

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