Porcine interferon-gamma (PoIFN-gamma) fused with glutathione S-transferase (GST) was expressed in Escherichia coli BL(21). Twenty 6-week-old piglets were randomly assigned to four groups. Pigs in groups 1-3 were pretreated with 30mg, 20mg, and 10mg recombinant PoIFN-gamma (rPoIFN-gamma), respectively. Pigs in group 4 (control) were pretreated with GST expressed by the empty plasmid. At 48h postinoculation (hpi), all swine were challenged with FMDV (serotype O). Pigs pretreated with 30mg rPoIFN-gamma were completely protected from virulent FMDV attack. Pigs given 20mg rPoIFN-gamma achieved partial protection, and the unprotected piglets showed clinical signs from 68h postchallenge (hpc). Although 10mg rPoIFN-gamma did not confer protection against FMDV, the pigs pretreated with this dose of rPoIFN-gamma presented clinical signs from 35hpc, which was later than the control group (14hpc). These results indicate that PoIFN-gamma can protect swine against attack from FMDV or delay the appearance of clinical signs; the effect is dose dependent.
Porcine interferon-gamma protects swine from foot-and-mouth disease virus (FMDV). Publishing Authors By Initials
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AFFILIATION: Laboratory of Animal Virology, College of Veterinary Medicine, Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province 430070, People's Republic of China.
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MEDLINETA: Vet Immunol Immunopathol
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