Pooling of case specimens to create standard serum sets for screening cancer biomarkers.

Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Research Abstract Details 

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Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Abstract Text:

Steven J Skates,Nora K Horick,Joseph M Moy,Anna M Minihan,Michael V Seiden,Jeffrey R Marks,Patrick Sluss,Daniel W Cramer,

BACKGROUND: Multiple identical sets of sera from cancer cases and controls would facilitate standardized testing of biomarkers. We describe the creation and use of standard serum sets developed from healthy donors and pooled sera from ovarian, breast, and endometrial cancer cases. METHODS: Two hundred seventy-five 0.3-mL aliquots of sera were created for each of the 95 healthy women, and residual serum was pooled to create 275 identical sets of 20 0.3-mL aliquots. Aliquots (1.0-1.5 mL) from 441 women were combined to create 12 breast and pelvic disease pools with at least 115 0.3-mL aliquots. Sets were assembled to contain aliquots from individual controls, replicates, and disease pools. Cancer antigens (CA), CA 125, CA 19.9, and CA 15.3, and carcinoembryonic antigen were measured in one set and in 217 women comprising six of the pelvic disease pools. Use of a set was illustrated for mesothelin (soluble mesothelin-related protein). Statistical output included concentration differences between pooled cases and controls (z values for single analytes; Mahalanobis distances for pairs), correlation between z values and sensitivities, coefficient of variations, and standardized biases. RESULTS: Marker concentrations in the six pelvic disease pools were generally within 0.25 SD of the actual average, and z values correlated well with sensitivities. CA 125 remains the best single marker for nonmucinous ovarian cancer, complemented by CA 15.3 or soluble mesothelin-related protein. There is no comparable breast cancer biomarker among the current analytes tested. CONCLUSION: The potential value of standard serum sets for initial assessment of candidate biomarkers is illustrated. Sets are now available through the Early Detection Research Network to evaluate biomarkers for women's cancers.

Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Publishing Authors By Initials

SJ Skates,NK Horick,JM Moy,AM Minihan,MV Seiden,JR Marks,P Sluss,DW Cramer,

For similar biological factors: biological markers: tumor markers, biological research abstracts see: biological factors: biological markers: tumor markers, biological research

PUBMED ID PMID:

MEDLINE DATE:

Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cancer epidemiology, biomarkers & prevention : a p

VOLUME: 16

Page Numbers: 334-41

Journal Abbreviation: Cancer Epidemiol. Biomarkers P

ISSN: 1055-9965

DAY: 3

MONTH: Feb

YEAR: 2007

Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9200608

Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Keywords Mesh Terms:

KEYWORDS: Tumor Markers, Biological

MESH TERMS: blood

Chemical & Substance for Abstract: Pooling of case specimens to create standard serum sets for screening cancer biomarkers. Information

Substance Name: Tumor Markers, Biological

Registry Number: 0

Grant and Affiliation Information for Pooling of case specimens to create standard serum sets for screening cancer biomarkers.

AFFILIATION: Biostatistics Center, Massachusetts General Hospital, Boston, USA.

Country: United States

AGENCY: United States NCI

GRANT: U01CA086381

ACRONYM: CA

MEDLINETA: Cancer Epidemiol Biomarkers Pr

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