Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill.

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Abstract Text:

S Vartak,M E Robbins,A A Spector,

Polyunsaturated fatty acids (PUFA) such as gamma-linolenic acid (GLA, 18:3n-6), eicosapentaenoic acid (EPA, 20:5n-3), and docosahexaenoic acid (DHA, 22:6n-3) have been shown to be cytotoxic to tumor cells. The objective of this work was to study the effects of PUFA on the radiation response of a 36B10 rat astrocytoma cell line. Supplementation of the astrocytoma cells with 15-45 microM GLA, EPA, or DHA produced marked changes in the fatty acid profiles of their phospholipids and neutral lipids. The methylene bridge index of these lipids increased significantly. These PUFA also exerted cytotoxic effects, as determined using the clonogenic cell survival assay. While GLA and DHA produced a moderate cell-killing effect, EPA was extremely cytotoxic, especially at a concentration of 45 microM. The monounsaturated oleic acid (OA, 18:1n-9) did not affect cell survival. Further, all three PUFA, and particularly GLA, increased the radiation-induced cell kill; OA did not enhance the effect of radiation. alpha-Tocopherol acetate blocked the enhanced radiation sensitivity of GLA- and DHA-supplemented cells. In conclusion, GLA, EPA, and DHA supplementation prior to, during, and after irradiation can enhance the radiation-induced cytotoxicity of rat astrocytoma cells. GLA and DHA supplementation post-irradiation also enhanced the radiation response of the 36B10 cells. Because GLA maximally increases the radioresponsiveness of a rat astrocytoma, this PUFA might prove useful in increasing the therapeutic efficacy of radiation in the treatment of certain gliomas.

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Publishing Authors By Initials

S Vartak,ME Robbins,AA Spector,

For similar lipids: fatty acids: fatty acids, unsaturated: fatty acids, essential: linolenic acids: gamma-linolenic acid research abstracts see: lipids: fatty acids: fatty acids, unsaturated: fatty acids, essential: linolenic acids: gamma-linolenic acid research

PUBMED ID PMID:

MEDLINE DATE:

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Lipids

VOLUME: 32

Page Numbers: 283-92

Journal Abbreviation: Lipids

ISSN: 0024-4201

DAY: 19

MONTH: Mar

YEAR: 1997

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 60450

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Keywords Mesh Terms:

KEYWORDS: gamma-Linolenic Acid

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill. Information

Substance Name: tocopherol acetate

Registry Number: 7695-91-2

Grant and Affiliation Information for Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill.

AFFILIATION: Department of Radiology, University of Iowa, Iowa City 52242, USA.

Country: UNITED STATES

AGENCY: United States NCI

GRANT: CA 66081

ACRONYM: CA

MEDLINETA: Lipids

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill Related Publications

• 20-Hydroxyeicosatetraenoic acid is a potent dilator of mouse basilar artery: role of cyclooxygenase.
• 20-carboxy-arachidonic acid is a dual activator of peroxisome proliferator-activated receptors alpha and gamma.
• 807C/T polymorphism in platelet glycoprotein Ia gene is not associated with retinal vein occlusion.
• A psychophysical and electrophysiological analysis of salt taste in Trpv1 null mice.
• Behavioral discrimination between sucrose and other natural sweeteners in mice: implications for the neural coding of T1R ligands.
• DNA immunization using highly conserved murine cytomegalovirus genes encoding homologs of human cytomegalovirus UL54 (DNA polymerase) and UL105 (helicase) elicits strong CD8 T-cell responses and is protective against systemic challenge.
• Efavirenz pharmacokinetics in HIV-1-infected children are associated with CYP2B6-G516T polymorphism.
• Fidelity of whole-genome amplification of blood spot DNA for HLA typing and SNP analyses.
• Giant intradiploic epidermoid cyst of greater sphenoid wing causing unilateral proptosis and optic nerve compression.
• Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants.
• Impact of nucleoside reverse transcriptase inhibitors on mitochondria in human immunodeficiency virus type 1-infected children receiving highly active antiretroviral therapy.
• Lifestyle behaviors in women with a BRCA1 or BRCA2 genetic mutation: an exploratory study guided by concepts derived from the Health Belief Model.
• Nuclear export of the human cytomegalovirus tegument protein pp65 requires cyclin-dependent kinase activity and the Crm1 exporter.
• Outer hair cell active force generation in the cochlear environment.
• Oxygenation of omega-3 fatty acids by human cytochrome P450 4F3B: effect on 20-hydroxyeicosatetraenoic acid production.
• Pediatric sinusitis.
• Performance of rapid HIV testing using Determine HIV-1/2 for the diagnosis of HIV infection during pregnancy in Tijuana, Baja California, Mexico.
• Polyunsaturated fatty acids increase the sensitivity of 36B10 rat astrocytoma cells to radiation-induced cell kill.
• Prevalence of primary HIV-1 drug resistance in pregnant women and in newly diagnosed adults at Tijuana General Hospital, Baja California, Mexico.
• Protein phosphorylation and the nuclear organization of pre-mRNA splicing.
• Reduced telomere length in rheumatoid arthritis is independent of disease activity and duration.
• Reproductive history, infertility treatment, and the risk of acute leukemia in children with down syndrome: a report from the Children's Oncology Group.
• Service with a smile: do emotional intelligence, gender, and autonomy moderate the emotional labor process?
• Sigma(s)-Dependent carbon-starvation induction of pbpG (PBP 7) is required for the starvation-stress response in Salmonella enterica serovar Typhimurium.
• Statins as modulators of bone formation.
• Telomere length in leukocytes correlates with bone mineral density and is shorter in women with osteoporosis.
• The IE2 60-kilodalton and 40-kilodalton proteins are dispensable for human cytomegalovirus replication but are required for efficient delayed early and late gene expression and production of infectious virus.
• The Relative Effects of Transection of the Gustatory Branches of the Seventh and Ninth Cranial Nerves on NaCl Taste Detection in Rats.
• The epidemiology of childhood leukemia with a focus on birth weight and diet.
• The functional consequences of gustatory nerve regeneration as assessed behaviorally in a rat model.