Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin.

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Abstract Text:

Shin-Ichi Sato,Youngjoo Kwon,Shinji Kamisuki,Neeta Srivastava,Qian Mao,Yoshinori Kawazoe,Motonari Uesugi,

Identification of protein targets of bioactive small molecules has been a technical hurdle of chemical genetics. Here we report a polyproline-rod approach to isolating protein targets of small molecules from cell lysates. The results indicate that insertion of a long, rigid polyproline helix between a small-molecule bait and a biotin tag boosts the capacity of affinity purification and thereby permits isolation of low-abundance or low-affinity proteins. In the course of the proof-of-concept experiments, we isolated glyoxalase 1 (GLO1) as a new target of indomethacin, a widely used antiinflammatory drug. Molecular biological experiments suggest that inhibition of GLO1 enzyme activity is related to the clinically recognized beneficial side effects of the indomethacin family of nonsteroidal antiinflammatory drugs.

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Publishing Authors By Initials

S Sato,Y Kwon,S Kamisuki,N Srivastava,Q Mao,Y Kawazoe,M Uesugi,

For similar nucleic acids, nucleotides, and nucleosides: antisense elements (genetics): rna, antisense: rna, small interfering research abstracts see: nucleic acids, nucleotides, and nucleosides: antisense elements (genetics): rna, antisense: rna, small interfering research

PUBMED ID PMID:

MEDLINE DATE:

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 129

Page Numbers: 873-80

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 31

MONTH: Jan

YEAR: 2007

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Keywords Mesh Terms:

KEYWORDS: RNA, Small Interfering

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin. Information

Substance Name: Lactoylglutathione Lyase

Registry Number: EC 4.4.1.5

Grant and Affiliation Information for Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin.

AFFILIATION: The Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA109277

ACRONYM: CA

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin Related Publications

• A gene-expression inhibitor that targets an alpha-helix-mediated protein interaction.
• A wrench-shaped synthetic molecule that modulates a transcription factor-coactivator interaction.
• Activation and repression of the activity of a lead ribozyme by the combination of Pb2+ and Mg2+.
• Demonstration of partial erasing of picosecond temporal optical data by use of accumulated photon echoes.
• Dimerization and DNA binding facilitate alpha-helix formation of Max in solution.
• Direct expression of a synthetic gene in Escherichia coli: purification and physicochemical properties of human initiation factor 4E.
• Dopamine agonists and valvular heart disease in patients with Parkinson's disease: evidence and mystery.
• Effects of 2'-substituents of the first deoxyguanosine residue in the recognition sequence of EcoRI restriction endonuclease activity.
• Expression of a synthetic gene for human cap binding protein (human IF-4E) in Escherichia coli and fluorescence studies on interaction with mRNA cap structure analogues.
• External control of Her2 expression and cancer cell growth by targeting a Ras-linked coactivator.
• Hairpin structure of an RNA 28-mer, which contains a sequence of the enzyme component of a hammerhead ribozyme system: evidence for tandem G:A pairs that are not of side-by-side type.
• Microcalcifications of Breast Tissue: Appearance on Synchrotron Radiation Imaging with 6-{micro}m Resolution.
• Polynucleotides. XXI. Synthesis and properties of poly 1-deazaadenylic acid and poly 3-deazaadenylic acid.
• Polyproline-rod approach to isolating protein targets of bioactive small molecules: isolation of a new target of indomethacin.
• Properties of a hammerhead ribozyme with deletion of stem II.
• Properties of hepatitis delta virus ribozyme, which consists of three RNA oligomer strands.
• Small molecule transcription factor mimic.
• Structure, interactions and effects on activity of the 5'-terminal region of human telomerase RNA.
• Studies on RNA-protein interactions and enzyme activity by mutational analysis of telomerase.
• [A case of so-called carcinosarcoma of the esophagus]
• [Directions in educational studies. 7. On the study of methodology in teaching attitudes--various studies in psychology]
• [Discovering high-affinity ligands for proteins: SAR by NMR]
• [Identification of the cardiac beta-adrenergic receptor protein: solubilization and purification by affinity chromatography]
• [In-vivo activation of the p53 pathway by small-molecule antagonists of MDM2]
• [Role of LXRs in control of lipogenesis]