Polymorphism of the signaling molecule c-di-GMP.

Polymorphism of the signaling molecule c-di-GMP. Research Abstract Details 

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Polymorphism of the signaling molecule c-di-GMP. Abstract Text:

Zhaoying Zhang,Seho Kim,Barbara L Gaffney,Roger A Jones,

Using UV, CD, and NMR, we demonstrate that the important bacterial signaling molecule involved in biofilm formation, cyclic diguanosine monophosphate (c-di-GMP), exists as a mixture of five different but related structures in an equilibrium that is sensitive both to its concentration and to the metal present. At the lower concentrations used for UV and CD work (0.05-0.5 mM), Li(+), Na(+), Cs(+), and Mg(2+) favor a bimolecular self-intercalated structure, while K(+), Rb(+), and NH(4)(+) favor formation of one or more guanine quartet complexes as well. At the higher NMR concentrations ( approximately 30 mM), the bimolecular structures associate and rearrange to a mixture of all-syn and all-anti tetramolecular and octamolecular quartet complexes. With K(+) the octamolecular complexes predominate, while with Li(+) the tetramolecular and octamolecular quartet complexes are present in approximately equal amounts, along with the bimolecular structure. We also find that both guanine amino groups in c-di-GMP are essential for formation of the quartets, because substitution of inosine for one guanosine allows formation of only the bimolecular structure. Further, two molecules of c-di-GMP tethered together are constrained in such a way that limits their ability to form these quartet complexes. The polymorphism we describe may provide different options for this signaling molecule when performing its functions in a bacterial cell, with K(+) and its own local concentration controlling the equilibrium.

Polymorphism of the signaling molecule c-di-GMP. Publishing Authors By Initials

Z Zhang,S Kim,BL Gaffney,RA Jones,

For similar investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research abstracts see: investigative techniques: chemistry, analytical: photometry: spectrophotometry: spectrophotometry, ultraviolet research

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Polymorphism of the signaling molecule c-di-GMP. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 7015-24

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 31

MONTH: May

YEAR: 2006

Polymorphism of the signaling molecule c-di-GMP. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Polymorphism of the signaling molecule c-di-GMP. Keywords Mesh Terms:

KEYWORDS: Spectrophotometry, Ultraviolet

MESH TERMS: chemistry

Chemical & Substance for Abstract: Polymorphism of the signaling molecule c-di-GMP. Information

Substance Name: Cyclic GMP

Registry Number: 7665-99-8

Grant and Affiliation Information for Polymorphism of the signaling molecule c-di-GMP.

AFFILIATION: Department of Chemistry and Chemical Biology, Rutgers-The State University of New Jersey, 610 Taylor Road, Piscataway, NJ 08854, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: EB 002809

ACRONYM: EB

MEDLINETA: J Am Chem Soc

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