Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin.

Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Research Abstract Details 

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Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Abstract Text:

Biyun Wu,Bruce Gerlitz,Brian W Grinnell,Mark E Meyerhoff,

Multi-functional bilayer polymeric coatings are prepared with both controlled nitric oxide (NO) release and surface-bound active thrombomodulin (TM) alone or in combination with immobilized heparin. The outer-layer is made of CarboSil, a commercially available copolymer of silicone rubber (SR) and polyurethane (PU). The CarboSil is either carboxylated or aminated via an allophanate reaction with a diisocyanate compound followed by a urea-forming reaction between the generated isocyanate group of the polymer and the amine group of an amino acid (glycine), an oligopeptide (triglycine) or a diamine. The carboxylated CarboSil can then be used to immobilize TM through the formation of an amide bond between the surface carboxylic acid groups and the lysine residues of TM. Aminated CarboSil can also be employed to initially couple heparin to the surface, and then the carboxylic acid groups on heparin can be further used to anchor TM. Both surface-bound TM and heparin's activity are evaluated by chromogenic assays and found to be at clinically significant levels. The underlying NO release layer is made with another commercial SR-PU copolymer (PurSil) mixed with a lipophilic NO donor (N-diazeniumdiolated dibutylhexanediamine (DBHD/N(2)O(2))). The NO release rate can be tuned by changing the thickness of top coatings, and the duration of NO release at physiologically relevant levels can be as long as 2 weeks. The combination of controlled NO release as well as immobilized active TM and heparin from/on the same polymeric surface mimics the highly thromboresistant endothelium layer. Hence, such multifunctional polymer coatings should provide more blood-compatible surfaces for biomedical devices.

Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Publishing Authors By Initials

B Wu,B Gerlitz,BW Grinnell,ME Meyerhoff,

For similar membrane glycoproteins: platelet membrane glycoproteins: receptors, thrombin: thrombomodulin research abstracts see: membrane glycoproteins: platelet membrane glycoproteins: receptors, thrombin: thrombomodulin research

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MEDLINE DATE:

Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biomaterials

VOLUME: 28

Page Numbers: 4047-55

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 26

MONTH: 06

YEAR: 2007

Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100316

Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Keywords Mesh Terms:

KEYWORDS: Thrombomodulin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin. Information

Substance Name: Heparin

Registry Number: 9005-49-6

Grant and Affiliation Information for Polymeric coatings that mimic the endothelium: combining nitric oxide release with surface-bound active thrombomodulin and heparin.

AFFILIATION: Department of Chemistry, The University of Michigan, 930 North University Avenue, Ann Arbor, MI 48109-1055, USA.

Country: England

AGENCY: United States NICHD

GRANT: HD-015434

ACRONYM: HD

MEDLINETA: Biomaterials

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