Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation.

Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation. Research Abstract Details 

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Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation. Abstract Text:

Zhiyong Chen,Li Xu,Yuan Liang,Jianbin Wang,Meiping Zhao,Yuanzong Li,Zhiyong Chen,Li Xu,Yuan Liang,Jianbin Wang,Meiping Zhao,Yuanzong Li,

A supermacroporous monolithic cryogel was directly prepared by in situ cryo-copolymerization in a stainless steel cartridge (70mmx5.0mm I.D.) using methacrylic acid (MAA) as functional monomer and polyethylene glycol diacrylate (PEGDA) as crosslinker. The highly crosslinked (90%, molar ratio) poly(MAA-PEGDA) cryogel had more uniform supermacropores with a mean diameter of 25mum compared to the poly(acrylamide)-based cryogels. The viability of poly(MAA-PEGDA) cryogel as a medium was demonstrated for separations of lysozyme from chicken egg white (CEW) and water-soluble poly(N-isopropylacrylamide-co-3-(dimethylamino) propyl methacrylamide) (NIPAM-DMAPMA) nanoparticles from its crude reaction solution. The dynamic binding capacities of lysozyme and the polymeric nanoparticles were 4.51x10(-3)mumol/ml and 33.4mug/ml, respectively. The lysozyme recovered from the above separations had a purity of more than 85%, and retained 90% of its enzymatic activity.

Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation. Publishing Authors By Initials

Z Chen,L Xu,Y Liang,J Wang,M Zhao,Y Li,Z Chen,L Xu,Y Liang,J Wang,M Zhao,Y Li,

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Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. A

VOLUME: 1182

Page Numbers: 128-31

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ISSN: 0021-9673

DAY: 6

MONTH: 01

YEAR: 2008

Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation.

AFFILIATION: Beijing National Laboratory for Molecular Sciences (BNLMS), The Key Laboratory of Bioorganic Chemistry & Molecular Engineering, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

Country: Netherlands

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MEDLINETA: J Chromatogr A

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