Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells.

Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Research Abstract Details 

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Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Abstract Text:

Dominique Vautier,Veronique Karsten,Christophe Egles,Johanna Chluba,Pierre Schaaf,Jean-Claude Voegel,Joëlle Ogier,

The aim of this study was to evaluate polyelectrolyte multilayer films as interfaces for implants. Polyelectrolyte multilayers were built up with different terminating layers by alternate deposition of oppositely charged polyelectrolytes on which chondrosarcoma (HCS-2/8) cells were grown in the presence of serum. Films formed by an increasing number of layers were investigated. The terminating layer was made of one of the following polyelectrolytes: poly-sodium-4-styrenesulfonate (PSS), poly-L-glutamic acid (PGA), poly-allylamine hydrochloride (PAH), or poly(L-lysine) (PLL). Cell viability, inflammatory response, adherence, and cytoskeletal organization were studied. Induction of interleukin-8 (IL-8) secretion was detected on PAH and PLL ending polyelectrolyte films. Early cellular adherence was enhanced with PGA, PAH, PLL, and, to a lower extent, PSS terminating layers. Adherence was independent of the number of layers constituting the films. The presence of actin filaments and vinculin focal adhesion spots was observed on PSS or PAH ending films. They were respectively partially and totally absent on PGA and PLL terminating multilayer architectures. For PLL ending films, vinculin and actin organization was clearly dependent on the number of deposited layers. The results of this study suggest that PSS ending multilayered films constitute a good interfacial micro-environment at the material surface for HCS-2/8 cells.

Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Publishing Authors By Initials

D Vautier,V Karsten,C Egles,J Chluba,P Schaaf,JC Voegel,J Ogier,

For similar proteins: cytoskeletal proteins: vinculin research abstracts see: proteins: cytoskeletal proteins: vinculin research

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Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 13

Page Numbers: 713-32

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 2002

Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Information

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LANGUAGE: eng

NlmUniqueID: 9007393

Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Keywords Mesh Terms:

KEYWORDS: Vinculin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells. Information

Substance Name: Vinculin

Registry Number: 125361-02-6

Grant and Affiliation Information for Polyelectrolyte multilayer films modulate cytoskeletal organization in chondrosarcoma cells.

AFFILIATION: INSERM U 424, UFR Odontologie, Strasbourg, France. Dominique.Vautier@medicine.u-strasbg.fr

Country: Netherlands

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MEDLINETA: J Biomater Sci Polym Ed

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