Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa.

Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa. Research Abstract Details 

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Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa. Abstract Text:

The subcellular localization of the major type II secretion system of Pseudomonas aeruginosa, the Xcp system, was studied microscopically using a biarsenical ligand that becomes fluorescent upon binding to a tetracysteine motif (Lumio tag), which was fused to several Xcp components. Fusion of the Lumio tag to the C termini of the XcpR and XcpS proteins did not affect the functionality of these proteins. Fluorescence microscopy showed that they were predominantly localized to the poles of P. aeruginosa cells, when produced at levels comparable to chromosomally encoded XcpR and XcpS. In most labelled cells, the proteins were found at one of the poles, although bipolar localization was also observed. When produced in the absence of other Xcp components, labelled XcpS was still found to locate at the poles, whereas XcpR was evenly distributed in the cell. These data suggest that XcpS, but not XcpR, contains information required for polar localization. The polar location of the Xcp machinery was further confirmed by the visualization of protease secretion with an intramolecularly quenched casein conjugate.

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Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Microbiology (Reading, England)

VOLUME: 154

Page Numbers: 3025-32

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 3

MONTH: Oct

YEAR: 2008

Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa.

AFFILIATION: Department of Molecular Microbiology and Institute of Biomembranes, Utrecht University, 3584 CH Utrecht, The Netherlands.

Country: England

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MEDLINETA: Microbiology

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