Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional.

Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional. Research Abstract Details 

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Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional. Abstract Text:

Suresh H Basagoudanavar,Xiaorong Feng,Gowdahalli Krishnegowda,Arivalagan Muthusamy,D Channe Gowda,Suresh H Basagoudanavar,Xiaorong Feng,Gowdahalli Krishnegowda,Arivalagan Muthusamy,D Channe Gowda,

The glycosylphosphatidylinositol (GPI) anchors of Plasmodium falciparum are indispensable for parasite survival since merozoite surface proteins-1, -2, -4, -5, and -10, crucial for erythrocyte invasion, are GPI-anchored. Therefore, the GPI biosynthetic pathway can offer potential targets for novel anti-malarial drugs. Here, we characterized the putative P. falciparum PIG-B gene (PfPIGB) that encodes mannosyltransferase-III of GPI biosynthesis. PfPIGB mRNA is transcribed in a developmental stage specific manner. A protein corresponding to the expected size of PfPIG-B is expressed by the parasite and is localized in the endoplasmic reticulum. Treatment of parasites with PfPIG-B specific siRNA caused reduction in GPI synthesis, affecting the PIG-B specific GPI intermediate. These data demonstrate that PfPIG-B is functional and encodes mannosyltransferase-III of the parasite GPI biosynthesis. The parasite PfPIG-B is novel in that its signature sequence HKEHKI is unique and is only partially conserved as compared to HKEXRF signature motif of mammalian PIG-B enzymes.

Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional. Publishing Authors By Initials

SH Basagoudanavar,X Feng,G Krishnegowda,A Muthusamy,DC Gowda,SH Basagoudanavar,X Feng,G Krishnegowda,A Muthusamy,DC Gowda,

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Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biochemical and biophysical research communication

VOLUME: 364

Page Numbers: 748-54

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 22

MONTH: 10

YEAR: 2007

Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional. Information

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LANGUAGE: eng

NlmUniqueID: 372516

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Grant and Affiliation Information for Plasmodium falciparum GPI mannosyltransferase-III has novel signature sequence and is functional.

AFFILIATION: Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI 41139

ACRONYM: AI

MEDLINETA: Biochem Biophys Res Commun

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