Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix.

Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Research Abstract Details 

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Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Abstract Text:

Hiroto Matsuoka,Thomas H Sisson,Teruaki Nishiuma,Richard H Simon,

Interventions that enhance plasminogen activation within the lung consistently limit the fibrosis that follows alveolar injury. However, this protective effect cannot be attributed solely to accelerated clearance of fibrin that forms as a provisional matrix after lung injury. To explore other mechanisms, we considered interactions between the plasminogen activation system and hepatocyte growth factor (HGF). HGF is known to have antifibrotic activity, but to do so, it must be both released from its sites of sequestration within extracellular matrix (ECM) and activated by proteolytic cleavage. A recent study using bleomycin-exposed mice showed that manipulations of the plasminogen activation system influenced the amount of free HGF within bronchoalveolar lavage fluid without affecting total lung HGF mRNA or protein. To elucidate the mechanisms, we studied the role of plasminogen activation in fibroblast-mediated HGF release and activation. We found that NIH3T3 and mouse lung fibroblasts release ECM-bound HGF in a plasminogen-dependent fashion. The plasminogen effect was lost when lung fibroblasts from urokinase-type plasminogen activator (uPA)-deficient mice were used, and was increased by fibroblasts from plasminogen activator inhibitor (PAI)-1-deficient mice. Plasminogen addition to NIH3T3 or mouse lung fibroblasts increased conversion of pro-HGF to its active form. The plasminogen effect on activation was lost when uPA-deficient fibroblasts were used and accentuated by PAI-1-deficient fibroblasts. In conjunction with the previous in vivo study, these results suggest that plasminogen activation can protect the lung against fibrosis by increasing the availability of active HGF.

Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Publishing Authors By Initials

H Matsuoka,TH Sisson,T Nishiuma,RH Simon,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: urinary plasminogen activator research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: urinary plasminogen activator research

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Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of respiratory cell and molecular

VOLUME: 35

Page Numbers: 705-13

Journal Abbreviation: Am. J. Respir. Cell Mol. Biol.

ISSN: 1044-1549

DAY: 13

MONTH: 07

YEAR: 2006

Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Information

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LANGUAGE: eng

NlmUniqueID: 8917225

Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Keywords Mesh Terms:

KEYWORDS: Urinary Plasminogen Activator

MESH TERMS: metabolism

Chemical & Substance for Abstract: Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix. Information

Substance Name: Urinary Plasminogen Activator

Registry Number: EC 3.4.21.73

Grant and Affiliation Information for Plasminogen-mediated activation and release of hepatocyte growth factor from extracellular matrix.

AFFILIATION: Pulmonary/Critical Care Medicine Division, Department of Internal Medicine, University of Michigan Health Sciences Center, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: P50-HL-56402

ACRONYM: HL

MEDLINETA: Am J Respir Cell Mol Biol

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