Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates.

Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Research Abstract Details 

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Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Abstract Text:

Timothy J Johnson,Yvonne M Wannemuehler,Sara J Johnson,Catherine M Logue,David G White,Curt Doetkott,Lisa K Nolan,

Despite the critical role of plasmids in horizontal gene transfer, few studies have characterized plasmid relatedness among different bacterial populations. Recently, a multiplex PCR replicon typing protocol was developed for classification of plasmids occurring in members of the Enterobacteriaceae. Here, a simplified version of this replicon typing procedure which requires only three multiplex panels to identify 18 plasmid replicons is described. This method was used to screen 1,015 Escherichia coli isolates of avian, human, and poultry meat origin for plasmid replicon types. Additionally, the isolates were assessed for their content of several colicin-associated genes. Overall, a high degree of plasmid variability was observed, with 221 different profiles occurring among the 1,015 isolates examined. IncFIB plasmids were the most common type identified, regardless of the source type of E. coli. IncFIB plasmids occurred significantly more often in avian pathogenic E. coli (APEC) and retail poultry E. coli (RPEC) than in uropathogenic E. coli (UPEC) and avian and human fecal commensal E. coli isolates (AFEC and HFEC, respectively). APEC and RPEC were also significantly more likely than UPEC, HFEC, and AFEC to possess the colicin-associated genes cvaC, cbi, and/or cma in conjunction with one or more plasmid replicons. The results suggest that E. coli isolates contaminating retail poultry are notably similar to APEC with regard to plasmid profiles, with both generally containing multiple plasmid replicon types in conjunction with colicin-related genes. In contrast, UPEC and human and avian commensal E. coli isolates generally lack the plasmid replicons and colicin-related genes seen in APEC and RPEC, suggesting limited dissemination of such plasmids among these bacterial populations.

Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Publishing Authors By Initials

TJ Johnson,YM Wannemuehler,SJ Johnson,CM Logue,DG White,C Doetkott,LK Nolan,

For similar urogenital system research abstracts see: urogenital system research

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MEDLINE DATE:

Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied and environmental microbiology

VOLUME: 73

Page Numbers: 1976-83

Journal Abbreviation: Appl. Environ. Microbiol.

ISSN: 0099-2240

DAY: 2

MONTH: 02

YEAR: 2007

Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7605801

Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Keywords Mesh Terms:

KEYWORDS: Urogenital System

MESH TERMS: microbiology

Chemical & Substance for Abstract: Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Information

Substance Name: DNA, Bacterial

Registry Number: 0

Grant and Affiliation Information for Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates.

AFFILIATION: Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, 1802 Elwood Drive, VMRI #2, Iowa State University, Ames, IA 50011, USA.

Country: United States

AGENCY: United States NIAID

GRANT: U24 AI 50139

ACRONYM: AI

MEDLINETA: Appl Environ Microbiol

REFSOURCE: Appl Environ Microbiol. 2007 Jun;73(11):

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