Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis.

Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis. Research Abstract Details 

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Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis. Abstract Text:

Patrick C Y Woo,Shirley S L Ma,Jade L L Teng,Maria W S Li,Susanna K P Lau,Kwok-yung Yuen,Patrick C Y Woo,Shirley S L Ma,Jade L L Teng,Maria W S Li,Susanna K P Lau,Kwok-yung Yuen,

Among 21 human strains of Laribacter hongkongensis, small plasmids were observed in four strains, and large ones in six strains. The smallest, 3264-bp plasmid, pHLHK19, has only one ORF that encodes a putative replication initiator protein and a predicted origin of replication (ori) with a DnaA box, three 18-bp direct repeats and five pairs of inverted repeats. An Escherichia coli-L. hongkongensis shuttle vector was constructed by ligating the HindIII-digested pHLHK19, containing the replication initiator protein and ori of pHLHK19, to HindIII-digested pBK-CMV. This shuttle vector can propagate in E. coli and L. hongkongensis with good transformation efficiencies.

Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis. Publishing Authors By Initials

PC Woo,SS Ma,JL Teng,MW Li,SK Lau,KY Yuen,PC Woo,SS Ma,JL Teng,MW Li,SK Lau,KY Yuen,

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Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biotechnology letters

VOLUME: 29

Page Numbers: 1575-82

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 4

MONTH: 07

YEAR: 2007

Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis.

AFFILIATION: State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Room 423, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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