Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS.

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Abstract Text:

Yue Zhang,Qing Deng,Jaclyn A Porath,Carol L Williams,Kristin J Pederson-Gulrud,Joseph T Barbieri,

Pseudomonas aeruginosa ExoS (453 amino acids) is a bifunctional type III cytotoxin, comprising a Rho GTPase-activating protein domain (RhoGAP), and a 14-3-3 dependent ADP-ribosyltransferase domain. In addition, ExoS contains a membrane localization domain (termed MLD, residues 51-77) which localizes and traffics ExoS within intoxicated host cells. While membrane localization has been shown to be essential for ExoS to ADP-ribosylate Ras, the relationship between intracellular localization and expression of RhoGAP activity has not been addressed. In this study, loss of MLD function was observed to abolish expression of ExoS RhoGAP activity in HeLa cells. One mutation within the MLD (R56, R63, D70 mutated to N, RRD-->N) diminished plasma membrane localization and altered the cell rounding phenotype elicited by ExoS RhoGAP. In addition, cell rounding caused by ExoS-MLD(RRD-->N) was reversed by dominant active Rac1, but not dominant active Cdc42, indicating a switch in ExoS RhoGAP substrate specificity. Mutation of the C-terminal polybasic region abolished the ability of dominant active Rac1 to protect HeLa cells from expression of the RhoGAP activity of ExoS-MLD(RRD-->N). This study shows the importance of membrane localization in the targeting of Rho GTPases by ExoS RhoGAP.

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Publishing Authors By Initials

Y Zhang,Q Deng,JA Porath,CL Williams,KJ Pederson-Gulrud,JT Barbieri,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cellular microbiology

VOLUME: 9

Page Numbers: 2192-201

Journal Abbreviation: Cell. Microbiol.

ISSN: 1462-5814

DAY: 8

MONTH: 05

YEAR: 2007

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100883691

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS.

AFFILIATION: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM069700

ACRONYM: GM

MEDLINETA: Cell Microbiol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS Related Publications

• A DENTAL STUDENT LOOKS AT THE PROFESSION.
• Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags.
• Antinociceptive activity of the N-methyl-D-aspartate receptor antagonist N-(2-Indanyl)-glycinamide hydrochloride (CHF3381) in experimental models of inflammatory and neuropathic pain.
• Association of botulinum neurotoxin serotypes a and B with synaptic vesicle protein complexes.
• Characterization of highly toxic type 2 ribosome-inactivating proteins from Adenia lanceolata and Adenia stenodactyla (Passifloraceae).
• Chromosomal localization and molecular characterization of three different 5S ribosomal DNA clusters in the sea urchin Paracentrotus lividus.
• Chromosome fragility in cattle with chronic enzootic haematuria.
• Comparison of operative and conservative treatment of fractures of the lower end of the femur involving the knee.
• Developmental origins of health and disease (DOHaD).
• Effects of cardiac resynchronization therapy on insulin-like growth factor-1 in patients with advanced heart failure.
• Factors associated with infant and adolescent mortality.
• Fat graft myringoplasty after unsuccessful tympanic membrane repair.
• Immunization with the cysteine proteinase Ldccys1 gene from Leishmania (Leishmania) chagasi and the recombinant Ldccys1 protein elicits protective immune responses in a murine model of visceral leishmaniasis.
• Intracellular localization of type III-delivered Pseudomonas ExoS with endosome vesicles.
• Intracellular trafficking of Pseudomonas ExoS, a type III cytotoxin.
• Local and systemic control in Ewing's sarcoma of the femur treated with chemotherapy, and locally by radiotherapy and/or surgery.
• Mechanism of substrate recognition by botulinum neurotoxin serotype A.
• Multiple pocket recognition of SNAP25 by botulinum neurotoxin serotype E.
• Multivariate classification and modeling in surface water pollution estimation.
• PSYCHOLOGY IN DENTAL PRACTICE.
• Plasma membrane localization affects the RhoGAP specificity of Pseudomonas ExoS.
• Racial inequalities and perinatal health in the southeast region of Brazil.
• Subunit Vaccine against the Seven Serotypes of Botulism.
• THYROGLOBULIN ANTIBODIES IN LEPROSY.
• The mortality impact of the August 2003 heat wave in France: Investigating the 'harvesting' effect and other long-term consequences.
• Why are the rates of cesarean section in Brazil higher in more developed cities than in less developed ones?
• [EVALUATION OF VARIOUS ENZYMATIC INDICES AND THE JIRGL TEST IN DIFFERENTIATION BETWEEN HEPATOCELLULAR AND CHOLOSTATIC JAUNDICE. II. APPLICATION OF DISCRIMINATING ANALYSES.]
• [Not Available.]
• [Not Available.]
• [Not Available.]