Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans.

Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Research Abstract Details 

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Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Abstract Text:

Olga Kinclova-Zimmermannova,Hana ,

The physiological role of Candida albicans Cnh1, a member of the Na+/H+ antiporter family, was characterized. Though CaCnh1p had broad substrate specificity and mediated efflux of at least four alkali metal cations upon heterologous expression in Saccharomyces cerevisiae, its presence in C. albicans cells was important especially for potassium homeostasis. In C. albicans, CaCnh1p tagged with GFP was localized in the plasma membrane of cells growing as both yeasts and hyphae. Deletion of CNH1 alleles did not affect tolerance to NaCl, LiCl or CsCl, but resulted in increased sensitivity to high external concentrations of KCl and RbCl. The potassium and rubidium tolerance of a cnh1 homozygous mutant was fully restored by reintegration of CNH1 into the genome. The higher sensitivity of the cnh1/cnh1 mutant to external KCl was caused by a lower K+ efflux from these cells. Together, the functional characterization of the CaCnh1 antiporter in C. albicans revealed that this antiporter plays a significant role in C. albicans physiology. It ensures potassium and rubidium tolerance and participates in the regulation of intracellular potassium content of C. albicans cells.

Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Publishing Authors By Initials

O Kinclova-Zimmermannova,H ,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 2603-12

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 27

MONTH: Aug

YEAR: 2007

Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Information

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LANGUAGE: eng

NlmUniqueID: 9430468

Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: chemistry

Chemical & Substance for Abstract: Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans. Information

Substance Name: rubidium chloride

Registry Number: 7791-11-9

Grant and Affiliation Information for Plasma-membrane Cnh1 Na+/H+ antiporter regulates potassium homeostasis in Candida albicans.

AFFILIATION: Department of Membrane Transport, Institute of Physiology AS CR, v.v.i., Videnska 1083, 142 20 Prague 4-Krc, Czech Republic.

Country: England

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MEDLINETA: Microbiology

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