Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton.

Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton. Research Abstract Details 

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Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton. Abstract Text:

Laura Vanagas,Rolando C Rossi,Ariel J Caride,Adelaida G Filoteo,Emanuel E Strehler,Juan Pablo F C Rossi,

Plasma membrane calcium pumps (PMCAs) are integral membrane proteins that actively expel Ca(2+) from the cell. Specific Ca(2+)-ATPase activity of erythrocyte membranes increased steeply up to 1.5-5 times when the membrane protein concentration decreased from 50 microg/ml to 1 microg/ml. The activation by dilution was also observed for ATP-dependent Ca(2+) uptake into vesicles from Sf9 cells over-expressing the PMCA 4b isoform, confirming that it is a property of the PMCA. Dilution of the protein did not modify the activation by ATP, Ca(2+) or Ca(2+)-calmodulin. Treatment with non-ionic detergents did not abolish the dilution effect, suggesting that it was not due to resealing of the membrane vesicles. Pre-incubation of erythrocyte membranes with Cytochalasin D under conditions that promote actin polymerization abolished the dilution effect. Highly-purified, micellar PMCA showed no dilution effect and was not affected by Cytochalasin D. Taken together, these results suggest that the concentration-dependent behavior of the PMCA activity was due to interactions with cytoskeletal proteins. The dilution effect was also observed with different PMCA isoforms, indicating that this is a general phenomenon for all PMCAs.

Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton. Publishing Authors By Initials

L Vanagas,RC Rossi,AJ Caride,AG Filoteo,EE Strehler,JP Rossi,

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Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 1768

Page Numbers: 1641-9

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 24

MONTH: 03

YEAR: 2007

Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton. Information

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LANGUAGE: eng

NlmUniqueID: 217513

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Grant and Affiliation Information for Plasma membrane calcium pump activity is affected by the membrane protein concentration: evidence for the involvement of the actin cytoskeleton.

AFFILIATION: IQUIFIB, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.

Country: Netherlands

AGENCY: United States FIC

GRANT: R03TW006837

ACRONYM: TW

MEDLINETA: Biochim Biophys Acta

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