Plasma cell purification from murine bone marrow using a two-step isolation approach.

Plasma cell purification from murine bone marrow using a two-step isolation approach. Research Abstract Details 

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Plasma cell purification from murine bone marrow using a two-step isolation approach. Abstract Text:

Heather A Minges Wols,Pamela L Witte,

Interest in plasma cells has increased greatly in the past decade. While several studies have examined the longevity and transcriptional control of antibody secreting cells in vivo, few studies have examined freshly isolated plasma cells ex vivo. Studies of primary plasma cells have been limited primarily due to the difficulty of isolating the large numbers of plasma cells necessary for experiments. In this protocol, plasma cells are purified from murine bone marrow in approximately 5 hours, following a two-step isolation method utilizing column enrichment and FACS-sorting based on CD138 (Syndecan-1) surface expression.

Plasma cell purification from murine bone marrow using a two-step isolation approach. Publishing Authors By Initials

HA Minges Wols,PL Witte,

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Plasma cell purification from murine bone marrow using a two-step isolation approach. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of immunological methods

VOLUME: 329

Page Numbers: 219-24

Journal Abbreviation: J. Immunol. Methods

ISSN: 0022-1759

DAY: 23

MONTH: 10

YEAR: 2007

Plasma cell purification from murine bone marrow using a two-step isolation approach. Information

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LANGUAGE: eng

NlmUniqueID: 1305440

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Grant and Affiliation Information for Plasma cell purification from murine bone marrow using a two-step isolation approach.

AFFILIATION: Science & Mathematics, Columbia College Chicago, 600 S. Michigan Avenue, Chicago, IL 60605 and Cell Biology, Neurobiology and Anatomy (Immunology and Aging Program), Loyola University Medical Center, 2160 S. 1st Avenue, Maywood, IL 60513, United States.

Country: Netherlands

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MEDLINETA: J Immunol Methods

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