PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis.

PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis. Research Abstract Details 

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PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis. Abstract Text:

Sara Abu-Ghanem,Galia Oberkovitz,Daniel Benharroch,Jacob Gopas,Etta Livneh,Sara Abu-Ghanem,Galia Oberkovitz,Daniel Benharroch,Jacob Gopas,Etta Livneh,

The Hodgkin-Reed-Sternberg (HRS) malignant cells in Hodgkin's lymphoma (HL) originate from germinal center B lymphocytes that did not undergo apoptosis. Protein Kinase C (PKC), a family of serine/threonine kinases, plays a crucial role in signal transduction modulating cell growth, differentiation and apoptosis. Here, we report the expression of PKC isoforms in two HL-derived cell lines, L428 and KMH2 and their correlation with drug resistance to CPT and doxorubicin. Among the PKC isoforms examined, only PKCeta and PKCbetaII were preferentially expressed in the drug resistant L428 cells. We have shown correlation between the response to apoptosis of L428 and KMH2 cells and PKCeta expression in these cell lines. In order to directly demonstrate a role for PKCeta in apoptosis, its expression was knocked-down by siRNA in the resistant L428 cells. Downregulation of PKCeta rendered L428 cells more sensitive to doxorubicin and CPT. Furthermore, PKCeta knocked-down cells showed increased PARP-1 cleavage, cytochrome c release and caspase 7 activation. It appears that PKCeta functions as an anti-apoptotic protein in HL-derived cell lines, and as we show here that it is also expressed in HRS of HL biopsies, it may have therapeutic relevance in HL. Thus, PKCeta could provide a new target aimed to reduce resistance to anti-cancer treatments of HL and other cancer patients.

PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis. Publishing Authors By Initials

S Abu-Ghanem,G Oberkovitz,D Benharroch,J Gopas,E Livneh,S Abu-Ghanem,G Oberkovitz,D Benharroch,J Gopas,E Livneh,

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PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cancer biology & therapy

VOLUME: 6

Page Numbers: 1375-80

Journal Abbreviation: Cancer Biol. Ther.

ISSN: 1555-8576

DAY: 1

MONTH: 06

YEAR: 2007

PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis. Information

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LANGUAGE: eng

NlmUniqueID: 101137842

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Grant and Affiliation Information for PKCeta expression contributes to the resistance of Hodgkin's lymphoma cell lines to apoptosis.

AFFILIATION: Department of Microbiology and Immunology, Faculty of Health Sciences, Ben-Gurion University, Beer-Sheva, Israel.

Country: United States

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MEDLINETA: Cancer Biol Ther

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