Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm.

Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm. Research Abstract Details 

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Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm. Abstract Text:

T Berger,

Fluorescein isothiocyanate-labelled pisum sativum agglutinin (FITC-PSA) was evaluated as an acrosomal stain for porcine and caprine sperm that had previously been stained with Hoechst 33258 to assess cell viability. The FITC-PSA procedure was as accurate as other procedures in assessing acrosomal presence or absence on either fresh or liquid-stored porcine sperm. Approximately half of the incubated porcine sperm with acrosomal loss maintained membrane impermeability to the Hoechst 33258; these were potentially viable acrosome-reacted sperm. The FITC-PSA procedure was significantly correlated with the assessment of acrosomal status of cryopreserved caprine sperm by transmission electron microscopy (TEM). However, TEM results indicated a higher percentage of caprine sperm with acrosomal loss. Ability to penetrate zona-free hamster ova was not highly correlated with the percentage of viable acrosome-reacted porcine or caprine sperm. The FITC-PSA procedure provides an estimate of viable acrosome reactions and may be a useful tool in the evaluation of sperm fertility.

Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm. Publishing Authors By Initials

T Berger,

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Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 33

Page Numbers: 689-95

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Mar

YEAR: 1990

Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Pisum sativum agglutinin used as an acrosomal stain of porcine and caprine sperm.

AFFILIATION: Department of Animal Science University of California, Davis, CA 95616 USA.

Country: United States

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MEDLINETA: Theriogenology

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