Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells.

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Abstract Text:

Jian Xu,Scott D Pope,Ali R Jazirehi,Joanne L Attema,Peter Papathanasiou,Jason A Watts,Kenneth S Zaret,Irving L Weissman,Stephen T Smale,

Recent studies have suggested that, in ES cells, inactive genes encoding early developmental regulators possess bivalent histone modification domains and are therefore poised for activation. However, bivalent domains were not observed at typical tissue-specific genes. Here, we show that windows of unmethylated CpG dinucleotides and putative pioneer factor interactions mark enhancers for at least some tissue-specific genes in ES cells. The unmethylated windows expand in cells that express the gene and contract, disappear, or remain unchanged in nonexpressing tissues. However, in ES cells, they do not always coincide with common histone modifications. Genomic footprinting and chromatin immunoprecipitation demonstrated that transcription factor binding underlies the unmethylated windows at enhancers for the Ptcra and Alb1 genes. After stable integration of premethylated Ptcra enhancer constructs into the ES cell genome, the unmethylated windows readily appeared. In contrast, the premethylated constructs remained fully methylated and silent after introduction into Ptcra-expressing thymocytes. These findings provide initial functional support for a model in which pioneer factor interactions in ES cells promote the assembly of a chromatin structure that is permissive for subsequent activation, and in which differentiated tissues lack the machinery required for gene activation when these ES cell marks are absent. The enhancer marks may therefore represent important features of the pluripotent state.

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Publishing Authors By Initials

J Xu,SD Pope,AR Jazirehi,JL Attema,P Papathanasiou,JA Watts,KS Zaret,IL Weissman,ST Smale,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 12377-82

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 18

MONTH: 07

YEAR: 2007

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells. Information

Substance Name: Interleukin-12

Registry Number: 187348-17-0

Grant and Affiliation Information for Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells.

AFFILIATION: Howard Hughes Medical Institute, Molecular Biology Institute, University of California, Los Angeles, CA 90095, USA.

Country: United States

AGENCY: United States NIAID

GRANT: T32 AI07323

ACRONYM: AI

MEDLINETA: Proc Natl Acad Sci U S A

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells Related Publications

• Associations between behavior, hormones, and Fos responses to novelty differ in pre- and post-pubertal grass rats.
• Combining a hybrid genetic algorithm and a heat transfer model to optimise an insulated box for use in the transport of perishables.
• Daily rhythms in PER1 within and beyond the suprachiasmatic nucleus of female grass rats (Arvicanthis niloticus).
• Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells.
• Predominant interaction of both Ikaros and Helios with the NuRD complex in immature thymocytes.
• Radiation measurements of the effluent from the Phoebus 1B reactor. LA-3787.
• The ultrastructure of peste des petits ruminants virus.
• Tyrosine hydroxylase positive neurons and their contacts with vasoactive intestinal polypeptide-containing fibers in the hypothalamus of the diurnal murid rodent, Arvicanthis niloticus.