Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells.

Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells. Research Abstract Details 

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Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells. Abstract Text:

Tao Sun,Bao-Cun Sun,Chun-Sheng Ni,Xiu-Lan Zhao,Xing-Hui Wang,Shuo Qie,Dan-Fang Zhang,Qiang Gu,Hong Qi,Nan Zhao,

Bone-marrow derived mesenchymal stem cells (BMSCs) have the potential to differentiate into osteocytes, chondrocytes, adipocytes and endothelial cells. The interaction between BMSCs and epithelial tumor cell was enhanced on proliferation. Our previous study had shown that BMSCs maybe participate in angiogenesis in melanoma in vivo. The aim of this study was to investigate the interaction between B16 melanoma cells and BMSCs in vitro, the mechanism of BMSCs participating in melanoma angiogenesis in vivo is unclear, so a co-culture system containing BMSCs and B16 melanoma cells, based on transwell indirect model, was established, and the interaction between BMSCs and B16 melanoma cells was studied in vitro. In our study, BMSCs were generated out of bone marrow from C57 mouse, isolated BMSCs were positive for the markers CD105, CD90, CD73, CD44 and CD166 and negative for endothelial markers, which acquired endothelial phenotype (including the expression of VEGFR-1, VEGFR-2, Factor VIII) after co-culture with B16 melanoma cells; at the same time, B16 melanoma cells also up-regulated the expression of VEGF-a, VEGFR-1, VEGFR-2 and Factor VIII. The proliferation rate of B16 melanoma cells and BMSCs were also found to be increased. We could show the differentiation of BMSCs into cells with phenotypic features of endothelial cells. BMSCs promoted proliferation of tumor cells and improved the microenvironment in tumor. Our study suggests that the BMSCs may play an important role in tumor angiogenesis.

Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells. Publishing Authors By Initials

T Sun,BC Sun,CS Ni,XL Zhao,XH Wang,S Qie,DF Zhang,Q Gu,H Qi,N Zhao,

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Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cancer letters

VOLUME: 263

Page Numbers: 35-43

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ISSN: 0304-3835

DAY: 30

MONTH: 01

YEAR: 2008

Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells. Information

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LANGUAGE: eng

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Country: Ireland

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MEDLINETA: Cancer Lett

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