Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae.

Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae. Research Abstract Details 

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Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae. Abstract Text:

Pengfei Xu,Yingpeng Han,Junjiang Wu,Huiying Lv,Lijuan Qiu,Ruzhen Chang,Limei Jin,Jinsheng Wang,Anliang Yu,Chen Chen,Haiyang Nan,Xiuhong Xu,Ping Wang,Dayong Zhang,Shuzhen Zhang,Wenbin Li,Weiyuan Chen,

The internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear ribosomal DNA (nrDNA) was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of the ITS of fungi. Around 800 bp-1,000 bp fragments were obtained based on the DL2000 marker and the sequences of the PCR products were tested. Taking isolate USA as outgroup, the phylogenetic tree was constructed by means of maximum parsimony analysis, and the genetic evolution among isolates was analyzed. The results showed that there is a great difference between the base constitution of ITS1 and ITS2 among various isolates. The seventeen isolates are classified into three groups, and the isolates from the same region belong to the same group, which shows the variation in geography.

Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae. Publishing Authors By Initials

P Xu,Y Han,J Wu,H Lv,L Qiu,R Chang,L Jin,J Wang,A Yu,C Chen,H Nan,X Xu,P Wang,D Zhang,S Zhang,W Li,W Chen,

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Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of genetics and genomics = Yi chuan xue ba

VOLUME: 34

Page Numbers: 180-8

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ISSN: 1673-8527

DAY: 1

MONTH: Feb

YEAR: 2007

Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Phylogenetic analysis of the sequences of rDNA internal transcribed spacer (ITS) of Phytophthora sojae.

AFFILIATION: Soybean Research Institute of Northeast Agricultural University, Key Laboratory of Soybean Biology of Education Department, Harbin 150030, China.

Country: China

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MEDLINETA: J Genet Genomics

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