Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays.

Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays. Research Abstract Details 

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Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays. Abstract Text:

Taek Jin Baek,Nam Hyun Kim,Jaebum Choo,Eun Kyu Lee,Gi Hun Seong,Taek Jin Baek,Nam Hyun Kim,Jaebum Choo,Eun Kyu Lee,Gi Hun Seong,

We describe the fabrication of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures with a high aspect ratio and the use of hydrogel microstructures containing the enzyme beta-galactosidase (beta-Gal) or glucose oxidase (GOx)/horseradish peroxidase (HRP) as biosensing components for the simultaneous detection of multiple analytes. The diameters of the hydrogel microstructures were almost the same at the top and at the bottom, indicating that no differential curing occurred through the thickness of the hydrogel microstructure. Using the hydrogel microstructures as microreactors, beta-Gal or GOx/HRP was trapped in the hydrogel array, and the time-dependent fluorescence intensities of the hydrogel array were investigated to determine the dynamic uptake of substrates into the PEG-DA hydrogel. The time required to reach steady-state fluorescence by glucose diffusing into the hydrogel and its enzymatic reactions with GOx and HRP was half the time required for resorufin beta-D-galactopyranoside (RGB) when used as the substrate for beta-Gal. Spatially addressed hydrogel microarrays containing different enzymes were micropatterned for the simultaneous detection of multiple analytes, and glucose and RGB solutions were incubated as substrates. These results indicate that there was no cross-talk between the beta-Gal-immobilizing hydrogel micropatches and the GOx/HRP-immobilizing micropatches.

Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays. Publishing Authors By Initials

TJ Baek,NH Kim,J Choo,EK Lee,GH Seong,TJ Baek,NH Kim,J Choo,EK Lee,GH Seong,

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Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of microbiology and biotechnology

VOLUME: 17

Page Numbers: 1826-32

Journal Abbreviation: J. Microbiol. Biotechnol.

ISSN: 1017-7825

DAY: 20

MONTH: Nov

YEAR: 2007

Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays. Information

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LANGUAGE: eng

NlmUniqueID: 9431852

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Grant and Affiliation Information for Photolithographic fabrication of poly(ethylene glycol) microstructures for hydrogel-based microreactors and spatially addressed microarrays.

AFFILIATION: Department of Applied Chemistry, Hanyang University, Ansan 425-791, Korea.

Country: Korea (South)

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MEDLINETA: J Microbiol Biotechnol

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