Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy.

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Abstract Text:

Ida Lee,Elias Greenbaum,Stephen Budy,Jason R Hillebrecht,Robert R Birge,Jeffrey A Stuart,

We report the direct measurement of photoinduced surface potential differences of wild-type (WT) and mutant D96N bacteriorhodopsin (BR) membranes at pH 7 and 10.5. Atomic force microscopy (AFM) and scanning surface potential microscopy (SSPM) were used to measure the BR membrane with the extracellular side facing up. We present AFM and SSPM images of WT and mutant D96N in which the light-dark transition occurred in the mid-scan of a single BR membrane. Photosteady-state populations of the M state were generated to facilitate measurement in each sample. The photoinduced surface potential of D96N is 63 mV (peak to valley) at pH 10.5 and is 48 mV at pH 7. The photoinduced surface potential of WT is 37 mV at pH 10.5 and approximately 0 at pH 7. Signal magnitudes are proportional to the amount of M produced at each pH. The results indicated that the surface potentials were generated by photoformation of surface charges on the extracellular side of the membrane. Higher surface potential correlated with a longer lifetime of the charges. A mechanistic basis for these signals is proposed, and it is concluded that they represent a steady-state measurement of the B2 photovoltage.

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Publishing Authors By Initials

I Lee,E Greenbaum,S Budy,JR Hillebrecht,RR Birge,JA Stuart,

For similar natural sciences: chemistry: chemistry, physical: photochemistry research abstracts see: natural sciences: chemistry: chemistry, physical: photochemistry research

PUBMED ID PMID:

MEDLINE DATE:

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The journal of physical chemistry. B

VOLUME: 110

Page Numbers: 10982-90

Journal Abbreviation:

ISSN: 1520-6106

DAY: 8

MONTH: Jun

YEAR: 2006

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101157530

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Keywords Mesh Terms:

KEYWORDS: Photochemistry

MESH TERMS: genetics

Chemical & Substance for Abstract: Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy. Information

Substance Name: Bacteriorhodopsins

Registry Number: 53026-44-1

Grant and Affiliation Information for Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy.

AFFILIATION: Department of Electrical and Computer Engineering, University of Tennessee, Knoxville, Tennessee 37996-2100, USA. ilee1@utk.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM-34548

ACRONYM: GM

MEDLINETA: J Phys Chem B

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy Related Publications

• An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei.
• An improved replica-exchange sampling method: Temperature intervals with global energy reassignment.
• Arm numbness and weakness after recurrent shoulder dislocation: 1028: may 30 3:35 PM - 3:55 PM.
• Book review: Cancer chemotherapy and biological response modifiers, Annual 17.
• Book review: Cancer in the elderly: A nursing and medical perspective.
• Can breast cancer be prevented?
• Comparative sequence analysis and tissue localization of members of the SLC6 family of transporters in adult Drosophila melanogaster.
• Dendritic mechanisms controlling spike-timing-dependent synaptic plasticity.
• Development and validation of the Inventory of Depression and Anxiety Symptoms (IDAS).
• Evidence-based medicine for the new millennium: Critical appraisal and pragmatic approach.
• Historical reflections on the afterhyperpolarization--firing rate relation of vertebrate spinal neurons.
• IGF-1 controls GLUT3 expression in muscle via the transcriptional factor Sp1.
• Interstrain inseminations and embryo transfers between the SLA miniature pig and standard crossbred pig.
• Isolation and compositional analysis of trypanosomatid editosomes.
• On deanships: survival tips for a hardship tour of duty.
• Oxa1 directly interacts with Atp9 and mediates its assembly into the mitochondrial F1Fo-ATP synthase complex.
• Photoinduced surface potential change of bacteriorhodopsin mutant D96N measured by scanning surface potential microscopy.
• Putting beta-diversity on the map: broad-scale congruence and coincidence in the extremes.
• RNA editing in Trypanosoma brucei requires three different editosomes.
• Recommending pathway genes using a compendium of clustering solutions.
• Response of dairy heifers to Prostaglandin F(2alpha) after treatment with human chorionic gonadotropin(1).
• The adolescent.
• The phylogenetic problem of Huia (Amphibia: Ranidae).
• The prevalence of postpartum depression: the relative significance of three social status indices.
• The teaching of the use of the polarizing microscope.
• Uridine insertion/deletion editing activities.
• Variation in incidence of pneumococcal and meningococcal disease across Europe.
• Wormlike aggregates from a supramolecular coordination polymer and a diblock copolymer.
• [Not Available.]
• [Not Available.]