Photo-oxidation of Jack bean urease in the presence of methylene blue.

Photo-oxidation of Jack bean urease in the presence of methylene blue. Research Abstract Details 

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Photo-oxidation of Jack bean urease in the presence of methylene blue. Abstract Text:

K Sakaguchi,K Mitsui,K Kobashi,J Hase,

Photo-oxidation of Jack bean urease was performed in the presence of a low concentration of methylene blue, which led to the complete loss of the enzymatic activity. The inactivation was more remarkable in an alkaline region than in an acidic region and prevented by the addition of histidine or methionine. Amino acid analysis of the oxidized enzyme revealed that the number of histidine residues had decreased to 73% that of the native enzyme, but the numbers of other amino acid residues were not significantly affected. Benzohydroxamic acid, a specific urease inhibitor, protected the active site of the enzyme against photo-oxidation. On the other hand, oxidation of the enzyme decreased its binding ability with caprylo- and benzohydroxamic acid to one-third. These results suggest that histidine residues are modified by photo-oxidation and are essential to both the enzymatic activity and the binding ability with hydroxamic acid.

Photo-oxidation of Jack bean urease in the presence of methylene blue. Publishing Authors By Initials

K Sakaguchi,K Mitsui,K Kobashi,J Hase,

For similar enzymes and coenzymes: enzymes: hydrolases: amidohydrolases: urease research abstracts see: enzymes and coenzymes: enzymes: hydrolases: amidohydrolases: urease research

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Photo-oxidation of Jack bean urease in the presence of methylene blue. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 93

Page Numbers: 681-6

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1983

Photo-oxidation of Jack bean urease in the presence of methylene blue. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Photo-oxidation of Jack bean urease in the presence of methylene blue. Keywords Mesh Terms:

KEYWORDS: Urease

MESH TERMS: radiation effects

Chemical & Substance for Abstract: Photo-oxidation of Jack bean urease in the presence of methylene blue. Information

Substance Name: Urease

Registry Number: EC 3.5.1.5

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Country: JAPAN

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MEDLINETA: J Biochem

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